I. Information of ICE

ICEberg ID	191
Name	10270-RD.2
Organism	Streptococcus pyogenes MGAS10270
Size (bp)	34300
GC content [Genome] (%)	34.63[38.43]
Insertion site	-
Function	Virulence: encodes two cell surface anchored adhesins; Spy1399 and Spy1410.
Species that ICE can be transferred to	-
Nucleotide Sequence	CP000260 (complete ICE sequence in this genome)
Replicon	chromosome (1928252 bp, BioProject:16364) [NC_008022]
Genome coordinates	1312338..1346637
Putative oriT region	-
Putative relaxase	coordinates: 1326499..1327740; Locus tag: MGAS10270_Spy1395;  Family:  MOBT

II. ICE interaction with IME/CIME/

The interaction information of 10270-RD.2 is not available.

The graph information of 10270-RD.2 components from CP000260
Complete gene list of 10270-RD.2 from CP000260
#	Gene	Coordinates [+/-], size (bp)	Product  (GenBank annotation)	*Reannotation
1	valS	1307544..1310192 [-], 2649	Valyl-tRNA synthetase
2	MGAS10270_Spy1374	1310194..1310778 [-], 585	hypothetical protein
3	MGAS10270_Spy1375	1310754..1310933 [-], 180	Ribosomal-protein-serineacetyltransferase
4	MGAS10270_Spy1376	1311358..1311753 [-], 396	hypothetical cytosolic protein
5	MGAS10270_Spy1377	1311771..1312025 [-], 255	hypothetical protein
6	int3	1312338..1313474 [-], 1137	DNA integration/recombination/inversion protein	Integrase
7	MGAS10270_Spy1379	1313524..1313856 [-], 333	hypothetical protein
8	MGAS10270_Spy1380	1314023..1314565 [-], 543	hypothetical membrane spanning protein
9	MGAS10270_Spy1381	1314788..1315549 [-], 762	Cell surface protein	PrgC, T4SS component
10	MGAS10270_Spy1382	1315574..1316131 [-], 558	hypothetical exported protein
11	MGAS10270_Spy1383	1316131..1317489 [-], 1359	hypothetical exported protein
12	MGAS10270_Spy1384	1317624..1317767 [+], 144	hypothetical protein
13	MGAS10270_Spy1385	1317935..1320250 [-], 2316	Membrane protein	YddG, T4SS component
14	MGAS10270_Spy1386	1320265..1322766 [-], 2502	DNA segregation ATPase	YddE, T4SS component
15	MGAS10270_Spy1387	1322787..1323197 [-], 411	hypothetical protein	YddD, T4SS component
16	MGAS10270_Spy1388	1323200..1323469 [-], 270	hypothetical membrane spanning protein
17	MGAS10270_Spy1389	1323488..1324438 [-], 951	hypothetical protein	YddB, T4SS component
18	MGAS10270_Spy1390	1324439..1324918 [-], 480	hypothetical protein
19	MGAS10270_Spy1391	1324926..1325177 [-], 252	hypothetical protein
20	MGAS10270_Spy1392	1325207..1325416 [-], 210	hypothetical protein
21	MGAS10270_Spy1393	1325460..1325732 [-], 273	hypothetical protein
22	MGAS10270_Spy1394	1326121..1326498 [-], 378	hypothetical cytosolic protein
23	MGAS10270_Spy1395	1326499..1327740 [-], 1242	Transcriptional regulator, Cro/CI family	Relaxase, MOBT Family
24	MGAS10270_Spy1396	1328052..1329761 [-], 1710	FtsK/SpoIIIE family	YdcQ, T4SS component
25	MGAS10270_Spy1397	1329781..1330236 [-], 456	hypothetical cytosolic protein
26	MGAS10270_Spy1398	1330239..1330544 [-], 306	hypothetical cytosolic protein
27	MGAS10270_Spy1399	1330806..1334864 [-], 4059	Cell surface protein	PrgB, T4SS component
28	MGAS10270_Spy1400	1335034..1337274 [-], 2241	M protein	PrgA, T4SS component
29	MGAS10270_Spy1401	1337367..1337759 [-], 393	hypothetical cytosolic protein
30	MGAS10270_Spy1402	1337883..1338323 [-], 441	Glutaredoxin-like protein
31	MGAS10270_Spy1403	1338481..1338915 [-], 435	Transcriptional regulator, Cro/CI family
32	MGAS10270_Spy1404	1339790..1340176 [+], 387	Transcriptional regulator, Cro/CI family
33	MGAS10270_Spy1405	1340183..1340605 [+], 423	hypothetical cytosolic protein
34	MGAS10270_Spy1406	1340670..1341026 [-], 357	hypothetical exported protein
35	MGAS10270_Spy1407	1341029..1341367 [-], 339	hypothetical cytosolic protein
36	MGAS10270_Spy1408	1341364..1341657 [-], 294	DNA-damage-inducible protein J
37	MGAS10270_Spy1409	1341678..1341863 [+], 186	Transposase
38	MGAS10270_Spy1410	1342369..1345203 [-], 2835	Cell surface protein
39	MGAS10270_Spy1411	1345432..1346637 [+], 1206	Transcriptional regulator, AraC family
40	MGAS10270_Spy1412	1347155..1347907 [+], 753	3-deoxy-7-phosphoheptulonate synthase
41	aroB	1347948..1349036 [+], 1089	3-dehydroquinate synthase
42	MGAS10270_Spy1414	1349306..1349464 [-], 159	hypothetical protein
43	MGAS10270_Spy1415	1349472..1349777 [-], 306	Putative acetate kinase
44	MGAS10270_Spy1416	1349779..1350117 [-], 339	hypothetical cytosolic protein
45	MGAS10270_Spy1417	1350170..1350925 [-], 756	SAM-dependent methyltransferase

Flanking regions

(1) Sitkiewicz I; Green NM; Guo N; Mereghetti L; Musser JM (2011). Lateral gene transfer of streptococcal ICE element RD2 (region of difference 2) encoding secreted proteins. BMC Microbiol. 0.503472222. [PubMed:21457552]

(2) Beres SB; Musser JM (2007). Contribution of exogenous genetic elements to the group A Streptococcus metagenome. PLoS One. 2(8):e800. [PubMed:17726530]

experimental literature