I. Information of ICE

ICEberg ID	181
Name	10750-RD.2
Organism	Streptococcus pyogenes MGAS10750
Size (bp)	48521
GC content [Genome] (%)	31.25
Insertion site	-
Function	Type II restriction-modification system; spectinomycin resistance; erythromycin Resistance
Species that ICE can be transferred to	-
Nucleotide Sequence	CP000262 (complete ICE sequence in this genome)
Replicon	chromosome (1937111 bp, BioProject:16366) [NC_008024]
Genome coordinates	1621728..1670248
Putative oriT region	-
Putative relaxase	coordinates: 1659807..1660178; Locus tag: MGAS10750_Spy1710;  Family:  MOBP

II. ICE interaction with IME/CIME/

The interaction information of 10750-RD.2 is not available.

The graph information of 10750-RD.2 components from CP000262
Complete gene list of 10750-RD.2 from CP000262
#	Gene	Coordinates [+/-], size (bp)	Product  (GenBank annotation)	*Reannotation
1	hsdS	1619589..1620764 [+], 1176	Type I restriction-modification system specificity subunit
2	MGAS10750_Spy1678	1620777..1621568 [+], 792	Type I restriction-modification system methylation subunit
3	MGAS10750_Spy1679	1621728..1622501 [+], 774	phage protein
4	MGAS10750_Spy1680	1622498..1623325 [+], 828	Replicative DNA helicase
5	MGAS10750_Spy1681	1623318..1623803 [+], 486	hypothetical protein
6	MGAS10750_Spy1682	1623800..1624534 [+], 735	phage antirepressor protein
7	MGAS10750_Spy1683	1624512..1626323 [+], 1812	TraG/TraD family
8	MGAS10750_Spy1684	1626342..1626617 [+], 276	hypothetical protein
9	MGAS10750_Spy1685	1626808..1627119 [+], 312	hypothetical protein
10	MGAS10750_Spy1686	1627121..1627336 [+], 216	hypothetical protein
11	MGAS10750_Spy1687	1627347..1628210 [+], 864	hypothetical protein
12	MGAS10750_Spy1688	1628220..1628510 [+], 291	hypothetical protein
13	MGAS10750_Spy1689	1628512..1628922 [+], 411	hypothetical protein
14	MGAS10750_Spy1690	1628813..1630831 [+], 2019	TrsE	T4CP
15	MGAS10750_Spy1691	1630788..1631237 [+], 450	TrsE	PrgJ, T4SS component
16	MGAS10750_Spy1692	1631245..1633824 [+], 2580	NlpC/P60 family protein	Orf14_Tn, T4SS component
17	MGAS10750_Spy1693	1633839..1634078 [+], 240	hypothetical protein
18	MGAS10750_Spy1694	1634065..1636416 [+], 2352	Putative surface protein
19	MGAS10750_Spy1695	1636431..1637300 [+], 870	hypothetical protein
20	MGAS10750_Spy1696	1637352..1639085 [+], 1734	DNA topoisomerase III
21	MGAS10750_Spy1697	1639078..1640043 [+], 966	Type II restriction-modification system methylation subunit
22	MGAS10750_Spy1698	1640030..1647673 [+], 7644	Superfamily II DNA and RNA helicase
23	MGAS10750_Spy1699	1647717..1648373 [+], 657	hypothetical protein
24	MGAS10750_Spy1700	1648925..1649557 [+], 633	Transcriptional regulator, TetR family
25	MGAS10750_Spy1701	1649642..1650523 [+], 882	Tetronasin resistance ATP-binding protein
26	MGAS10750_Spy1702	1650456..1652162 [+], 1707	Tetronasin resistance transmembrane protein
27	MGAS10750_Spy1704	1652743..1652949 [-], 207	hypothetical protein
28	MGAS10750_Spy1703	1652896..1653627 [+], 732	rRNA adenine N-6-methyltransferase
29	MGAS10750_Spy1705	1653844..1654368 [+], 525	spectinomycin phosphotransferase
30	MGAS10750_Spy1706	1654414..1654803 [+], 390	Cytidine deaminase
31	MGAS10750_Spy1707	1654782..1655486 [+], 705	Zn-dependent hydrolase
32	MGAS10750_Spy1708	1656371..1657465 [-], 1095	Relaxase
33	MGAS10750_Spy1709	1657466..1659343 [-], 1878	Reverse transcriptase / RNA maturase / Endonuclease
34	MGAS10750_Spy1710	1659807..1660178 [-], 372	Relaxase	Relaxase, MOBP Family
35	MGAS10750_Spy1711	1660180..1660638 [-], 459	hypothetical protein
36	MGAS10750_Spy1712	1660707..1661801 [-], 1095	RhuM
37	MGAS10750_Spy1713	1662273..1662824 [-], 552	phage-related protein
38	MGAS10750_Spy1714	1662833..1663234 [-], 402	Transcriptional regulator, Cro/CI family
39	MGAS10750_Spy1715	1663384..1663944 [+], 561	hypothetical protein
40	MGAS10750_Spy1716	1664213..1664623 [+], 411	hypothetical protein
41	MGAS10750_Spy1717	1665419..1667092 [+], 1674	Site-specific recombinase
42	MGAS10750_Spy1718	1667092..1668762 [+], 1671	Site-specific recombinase
43	MGAS10750_Spy1719	1668755..1670248 [+], 1494	Site-specific recombinase	Integrase
44	hsdM	1670421..1671224 [+], 804	Type I restriction-modification system methylation subunit
45	MGAS10750_Spy1721	1671605..1671724 [+], 120	hypothetical protein
46	salR	1671781..1672386 [-], 606	Transcriptional regulatory protein degU
47	MGAS10750_Spy1723	1672367..1673929 [-], 1563	Sensory transduction protein kinase

Flanking regions

(1) Sitkiewicz I; Green NM; Guo N; Mereghetti L; Musser JM (2011). Lateral gene transfer of streptococcal ICE element RD2 (region of difference 2) encoding secreted proteins. BMC Microbiol. 0.503472222. [PubMed:21457552]

(2) Brenciani A; Tiberi E; Bacciaglia A; Petrelli D; Varaldo PE; Giovanetti E (2011). Two distinct genetic elements are responsible for erm(TR)-mediated erythromycin resistance in tetracycline-susceptible and tetracycline-resistant strains of Streptococcus pyogenes. Antimicrob Agents Chemother. 55(5):2106-12. [PubMed:21343455]

(3) Beres SB; Musser JM (2007). Contribution of exogenous genetic elements to the group A Streptococcus metagenome. PLoS One. 2(8):e800. [PubMed:17726530]

experimental literature