 ICEberg ID | 163 |
Name  | SLP1 {AICE}  |
| ICEO ID | ICEO_0000748 |
| Organism | Streptomyces coelicolor A3(2) |
| Size (bp) | 17204 |
| GC content [Genome] (%) | 67.94[72.12] |
| Insertion site | tRNA-Tyr |
| Function | Cleavage of phosphorothioated DNA and methylated DNA by the type IV restriction endonuclease ScoMcrA |
| Species that ICE can be transferred to | Streptomyces lividans |
| Nucleotide Sequence | NC_003888 (complete ICE sequence in this genome) |
| Replicon | chromosome (8667507 bp, BioProject:242) [NC_003888] |
| Genome coordinates | 5038594..5055797 |
| Putative oriT region | - |
| Putative relaxase | - |
The graph information of SLP1 {AICE} components from NC_003888 | |||||
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| Complete gene list of SLP1 {AICE} from NC_003888 | |||||
| # | Gene | Coordinates [+/-], size (bp) | Product (GenBank annotation) | *Reannotation | |
| 1 | SCO4609 | 5033984..5034847 [+], 864 | heat shock protein HtpX | ||
| 2 | SCO4610 | 5034844..5035260 [+], 417 | hypothetical protein | ||
| 3 | SCO4611 | 5035358..5035951 [+], 594 | hypothetical protein | ||
| 4 | SCO4612 | 5036030..5037274 [-], 1245 | amino acid transporter | ||
| 5 | SCO4613 | 5037414..5037680 [+], 267 | integral membrane protein | ||
| 6 | SCO4614 | 5037788..5038276 [-], 489 | putative nucleotide-binding protein | ||
| 7 | SCO4615 | 5038758..5040125 [-], 1368 | integrase | Integrase | |
| 8 | SCO4616 | 5040186..5040371 [-], 186 | excisionase | ||
| 9 | SCO4617 | 5040809..5042281 [-], 1473 | ATP-binding protein | ||
| 10 | SCO4618 | 5042283..5043134 [-], 852 | hypothetical protein | ||
| 11 | SCO4619 | 5043224..5043691 [-], 468 | integral membrane protein | ||
| 12 | SCO4620 | 5043865..5045910 [-], 2046 | sporulation-related protein | T4CP | |
| 13 | SCO4621 | 5045925..5046464 [-], 540 | sporulation-related protein | ||
| 14 | SCO4622 | 5046484..5046984 [-], 501 | integral membrane protein | ||
| 15 | SCO4623 | 5047012..5047170 [+], 159 | hypothetical protein | ||
| 16 | SCO4624 | 5047328..5047663 [-], 336 | hypothetical protein | ||
| 17 | SCO4625 | 5047663..5048670 [-], 1008 | hypothetical protein | ||
| 18 | SCO4626 | 5048667..5049200 [-], 534 | hypothetical protein | ||
| 19 | SCO4627 | 5049197..5049466 [-], 270 | hypothetical protein | ||
| 20 | SCO4628 | 5049893..5050702 [+], 810 | regulator | ||
| 21 | SCO4629 | 5050699..5051364 [+], 666 | hypothetical protein | ||
| 22 | SCO4630 | 5051465..5052724 [-], 1260 | hypothetical protein | ||
| 23 | ScoMcrA(SCO4631) | 5052984..5054666 [-], 1683 | type IV restriction endonuclease ScoMcrA | ||
| 24 | SCO4632 | 5055828..5058728 [+], 2901 | ATP/GTP binding protein | ||
| 25 | SCO4633 | 5059025..5059681 [-], 657 | hypothetical protein | ||
 Flanking regions |
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(1) Liu G; Ou HY; Wang T; Li L; Tan H; Zhou X; Rajakumar K; Deng Z; He X (2010). Cleavage of phosphorothioated DNA and methylated DNA by the type IV restriction endonuclease ScoMcrA. PLoS Genet. 6(12):e1001253. [PubMed:21203499]
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(2) Brasch MA; Pettis GS; Lee SC; Cohen SN (1993). Localization and nucleotide sequences of genes mediating site-specific recombination of the SLP1 element in Streptomyces lividans. J Bacteriol. 175(10):3067-74. [PubMed:8387993]
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(3) Bibb MJ; Ward JM; Kieser T; Cohen SN; Hopwood DA (1981). Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividans. Mol Gen Genet. 184(2):230-40. [PubMed:6948998]
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(4) Omer CA; Cohen SN (1984). Plasmid formation in Streptomyces: excision and integration of the SLP1 replicon at a specific chromosomal site. Mol Gen Genet. 196(3):429-38. [PubMed:6094971]
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(5) Lee SC; Omer CA; Brasch MA; Cohen SN (1988). Analysis of recombination occurring at SLP1 att sites. J Bacteriol. 170(12):5806-13. [PubMed:3056916]
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(6) Vogtli M; Cohen SN (1992). The chromosomal integration site for the Streptomyces plasmid SLP1 is a functional tRNA(Tyr) gene essential for cell viability. Mol Microbiol. 6(20):3041-50. [PubMed:1479893]
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| experimental literature |