 ICEberg ID | 124 |
Name  | pRS01  |
| ICEO ID | ICEO_0000743 |
| Organism | Lactococcus lactis subsp. lactis ML3 |
| Size (bp) | - |
| GC content [Genome] (%) | 48.4 |
| Insertion site | - |
| Function | - |
| Species that ICE can be transferred to | - |
| Nucleotide Sequence | U50902 (partial ICE sequence) |
| Putative oriT region | - |
| Putative relaxase | coordinates: 6742..10925; Locus tag: -; Family: MOBP |
The graph information of pRS01 components from U50902 | |||||
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| Incomplete gene list of pRS01 from U50902 | |||||
| # | Gene | Coordinates [+/-], size (bp) | Product (GenBank annotation) | *Reannotation | |
| 1 | ltrM | 1386..3155 [+], 1770 | LtrM | ||
| 2 | ltrC | 3207..4859 [+], 1653 | LtrC | ||
| 3 | ltrD | 4932..5408 [+], 477 | LtrD | ||
| 4 | ltrE | 5639..6343 [+], 705 | LtrE | ||
| 5 | ltrF | 6386..6742 [+], 357 | LtrF | ||
| 6 | ltrB | 6742..10925 [+], 4184 | relaxase | Relaxase, MOBP Family | |
| 7 | ltrA | 7795..9594 [+], 1800 | putative maturase | ||
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(1) Belhocine K; Mandilaras V; Yeung B; Cousineau B (2007). Conjugative transfer of the Lactococcus lactis sex factor and pRS01 plasmid to Enterococcus faecalis. FEMS Microbiol Lett. 269(2):289-94. [PubMed:17263841]
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(2) Mills DA; McKay LL; Dunny GM (1996). Splicing of a group II intron involved in the conjugative transfer of pRS01 in lactococci. J Bacteriol. 178(12):3531-8. [PubMed:8655550]
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(3) Mills DA; Choi CK; Dunny GM; McKay LL (1994). Genetic analysis of regions of the Lactococcus lactis subsp. lactis plasmid pRS01 involved in conjugative transfer. Appl Environ Microbiol. 60(12):4413-20. [PubMed:7811081]
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| experimental literature |