I. Information of ICE
ICEberg ID123
Name pMEA300 {AICE} This ICE is derived from experimental literature
ICEO ID ICEO_0000741
OrganismAmycolatopsis methanolica NCIB11946
Size (bp)13285
GC content [Genome] (%)69.35
Insertion sitetRNA-Ile
Species that ICE can be transferred to-
Nucleotide SequenceL36679 (complete ICE sequence in this GenBank file)
Putative oriT region -
Putative relaxase -

II. ICE interaction with IME/CIME/

The interaction information of pMEA300 {AICE} is not available.

The graph information of pMEA300 {AICE} components from L36679
Complete gene list of pMEA300 {AICE} from L36679
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
1stf9879..11000 [+], 1122-

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
(1) te Poele EM; Samborskyy M; Oliynyk M; Leadlay PF; Bolhuis H; Dijkhuizen L (2008). Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded. Plasmid. 59(3):202-16. [PubMed:18295883] in_silico
(2) te Poele EM; Habets MN; Tan GY; Ward AC; Goodfellow M; Bolhuis H; Dijkhuizen L (2007). Prevalence and distribution of nucleotide sequences typical for pMEA-like accessory genetic elements in the genus Amycolatopsis. FEMS Microbiol Ecol. 61(2):285-94. [PubMed:17535299] experimental
(3) Vrijbloed JW; Jelinkova M; Hessels GI; Dijkhuizen L (1995). Identification of the minimal replicon of plasmid pMEA300 of the methylotrophic actinomycete Amycolatopsis methanolica. Mol Microbiol. 18(1):21-31. [PubMed:8596458] experimental
(4) Vrijbloed JW; Madon J; Dijkhuizen L (1995). Transformation of the methylotrophic actinomycete Amycolatopis methanolica with plasmid DNA: stimulatory effect of a pMEA300-encoded gene. Plasmid. 34(2):96-104. [PubMed:8559807] experimental
(5) Vrijbloed JW; Madon J; Dijkhuizen L (1994). A plasmid from the methylotrophic actinomycete Amycolatopsis methanolica capable of site-specific integration. J Bacteriol. 176(22):7087-90. [PubMed:7961475] experimental
experimental experimental literature
in_silico in silico analysis literature