I. Information of ICE

ICEberg ID	122
Name	pMEA100 {AICE}
ICEO ID	ICEO_0000740
Organism	Amycolatopsis mediterranei LBG A3136
Size (bp)	23290
GC content [Genome] (%)	68.44
Insertion site	tRNA-Phe
Function	Eliciting the lethal zygosis phenotype
Species that ICE can be transferred to	-
Nucleotide Sequence	EU149765 (complete ICE sequence in this GenBank file)
Replicon	-
Coordinates	1..23290
Putative oriT region	-
Putative relaxase	-

II. ICE interaction with IME/CIME/

The interaction information of pMEA100 {AICE} is not available.

The graph information of pMEA100 {AICE} components from EU149765
Complete gene list of pMEA100 {AICE} from EU149765
#	Gene	Coordinates [+/-], size (bp)	Product  (GenBank annotation)	*Reannotation
1	repAM	1..1113 [+], 1113	replication initiator protein
2	xis	1434..1736 [+], 303	excisionase
3	int	1752..3098 [+], 1347	bacteriophage-related integrase	Integrase
4	amp104	3394..3855 [+], 462	hypothetical protein
5	amp105	4891..5238 [+], 348	hypothetical protein
6	amp106	5552..6346 [-], 795	hypothetical protein
7	mdp	7116..7688 [+], 573	metal dependent phosphohydrolase
8	nud1	7680..8156 [-], 477	NUDIX hydrolase
9	amp109	8162..8911 [-], 750	XRE family transcriptional regulator
10	amp110	9084..9287 [+], 204	pMEA-specific protein
11	korA	9284..9631 [-], 348	GntR family transcriptional regulator
12	amp112	9752..10009 [+], 258	hypothetical protein
13	amp113	10318..11040 [+], 723	hypothetical protein
14	nud2	11081..11605 [+], 525	NUDIX hydrolase
15	ssgA	11623..11976 [+], 354	SsgA family transcriptional regulator
16	traF	12445..12675 [+], 231	TraF
17	traG	12675..12989 [+], 315	TraG
18	traH	12880..13314 [+], 435	TraH
19	traI	13311..14492 [+], 1182	TraI
20	traJ	14599..16911 [+], 2313	TraJ
21	amp121	17208..17654 [+], 447	hypothetical protein
22	amp122	17644..18387 [-], 744	hypothetical protein
23	ssb	18442..18924 [+], 483	single-strand DNA-binding protein
24	amp124	19233..20189 [+], 957	N-terminal primase-polymerase (primpol)-domain containing protein
25	amp125	20523..21023 [+], 501	hypothetical protein
26	amp126	21020..21373 [+], 354	hypothetical protein
27	dmt	21373..22998 [+], 1626	C-5 cytosine-specific DNA methylase

(1) te Poele EM; Samborskyy M; Oliynyk M; Leadlay PF; Bolhuis H; Dijkhuizen L (2008). Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded. Plasmid. 59(3):202-16. [PubMed:18295883]

(2) te Poele EM; Habets MN; Tan GY; Ward AC; Goodfellow M; Bolhuis H; Dijkhuizen L (2007). Prevalence and distribution of nucleotide sequences typical for pMEA-like accessory genetic elements in the genus Amycolatopsis. FEMS Microbiol Ecol. 61(2):285-94. [PubMed:17535299]

(3) Moretti P; Hintermann G; Hutter R (1985). Isolation and characterization of an extrachromosomal element from Nocardia mediterranei. Plasmid. 14(2):126-33. [PubMed:2999850]

(4) Madon J; Moretti P; Hutter R (1987). Site-specific integration and excision of pMEA100 in Nocardia mediterranei. Mol Gen Genet. 209(2):257-64. [PubMed:2823074]

experimental literature

in silico analysis literature