 ICEberg ID | 117 |
Name  | pSAM2 {AICE}  |
| ICEO ID | ICEO_0000745 |
| Organism | Streptomyces ambofaciens ATCC 15154 B3 |
| Size (bp) | 10.9 kb |
| GC content [Genome] (%) | - |
| Insertion site | tRNA-Pro |
| Function | Chromosome mobilising ability |
| Species that ICE can be transferred to | Streptomyces lividans |
| Nucleotide Sequence | AF004315; Z19593; X14899; Z19594; Z19589; A09946; M22966; M22965; M22964; M22963; AJ005260 (partial ICE sequence) |
| Putative oriT region | - |
| Putative relaxase | - |
The graph information of pSAM2 {AICE} components from A09946 | |||||
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| Incomplete gene list of pSAM2 {AICE} from A09946 | |||||
| # | Gene | Coordinates [+/-], size (bp) | Product (GenBank annotation) | *Reannotation | |
| 1 | xis | 88..291 [+], 204 | excision protein | ||
| 2 | int | 275..1441 [+], 1167 | integrase | Integrase | |
The graph information of pSAM2 {AICE} components from AJ005260 | |||||
 | |||||
| Incomplete gene list of pSAM2 {AICE} from AJ005260 | |||||
| # | Gene | Coordinates [+/-], size (bp) | Product (GenBank annotation) | *Reannotation | |
| 1 | orf131 | 503..898 [-], 396 | Orf131 | ||
| 2 | korSA | 986..1765 [+], 780 | KorSA | ||
| 3 | pra | 1905..2255 [+], 351 | Pra | ||
| 4 | traSA | 2366..3613 [+], 1248 | TraSA | TcpA, T4SS component | |
| 5 | spdA | 3696..4370 [+], 675 | SpdA | ||
| 6 | spdB | 4393..4569 [+], 177 | SpdB | ||
| 7 | spdC | 4579..4734 [+], 156 | SpdC | ||
| 8 | spdD | 4747..5061 [+], 315 | SpdD | ||
| 9 | orf183 | 5159..5710 [+], 552 | Orf183 | ||
| 10 | orf50 | 5804..5956 [-], 153 | - | ||
| 11 | repSA | 5971..7350 [+], 1380 | RepSA | ||
| 12 | xis | 7347..7535 [+], 189 | Xis | ||
| 13 | int | 7535..8701 [+], 1167 | Int (integrase) | Integrase | |
The graph information of pSAM2 {AICE} components from Z19594 | |||||
 | |||||
| Incomplete gene list of pSAM2 {AICE} from Z19594 | |||||
| # | Gene | Coordinates [+/-], size (bp) | Product (GenBank annotation) | *Reannotation | |
| 1 | - | 154..705 [+], 552 | Orf183 | ||
| 2 | - | 799..951 [-], 153 | Orf50 | ||
| 3 | - | 966..2345 [+], 1380 | RepSA | ||
| 4 | - | 2342..2530 [+], 189 | Xis | ||
The graph information of pSAM2 {AICE} components from Z19593 | |||||
 | |||||
| Incomplete gene list of pSAM2 {AICE} from Z19593 | |||||
| # | Gene | Coordinates [+/-], size (bp) | Product (GenBank annotation) | *Reannotation | |
| 1 | TraSA | 108..1355 [+], 1248 | - | TcpA, T4SS component | |
| 2 | SpdA | 1438..2112 [+], 675 | - | ||
| 3 | SpdB | 2135..2311 [+], 177 | - | ||
| 4 | SpdC | 2321..2476 [+], 156 | - | ||
| 5 | SpdD | 2489..2803 [+], 315 | - | ||
The graph information of pSAM2 {AICE} components from Z19589 | |||||
 | |||||
| Incomplete gene list of pSAM2 {AICE} from Z19589 | |||||
| # | Gene | Coordinates [+/-], size (bp) | Product (GenBank annotation) | *Reannotation | |
| 1 | - | 266..1045 [+], 780 | KorSA | ||
The graph information of pSAM2 {AICE} components from X14899 | |||||
 | |||||
| Incomplete gene list of pSAM2 {AICE} from X14899 | |||||
| # | Gene | Coordinates [+/-], size (bp) | Product (GenBank annotation) | *Reannotation | |
| 1 | - | 159..347 [+], 189 | - | ||
| 2 | - | 347..1513 [+], 1167 | - | Integrase | |
 Gene may contribute to site-specific recombination |
(1) te Poele EM; Samborskyy M; Oliynyk M; Leadlay PF; Bolhuis H; Dijkhuizen L (2008). Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded. Plasmid. 59(3):202-16. [PubMed:18295883] 
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(2) Possoz C; Gagnat J; Sezonov G; Guerineau M; Pernodet JL (2003). Conjugal immunity of Streptomyces strains carrying the integrative element pSAM2 is due to the pif gene (pSAM2 immunity factor). Mol Microbiol. 47(5):1385-93. [PubMed:12603742]
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(3) Sezonov G; Duchene AM; Friedmann A; Guerineau M; Pernodet JL (1998). Replicase, excisionase, and integrase genes of the Streptomyces element pSAM2 constitute an operon positively regulated by the pra gene. J Bacteriol. 180(12):3056-61. [PubMed:9620953]
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(4) Seoane A; Navas J; Garcia Lobo JM (1997). Targets for pSAM2 integrase-mediated site-specific integration in the Mycobacterium smegmatis chromosome. Microbiology. 143 ( Pt 10):3375-80. [PubMed:9353939]
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(5) Sezonov G; Hagege J; Pernodet JL; Friedmann A; Guerineau M (1995). Characterization of pra, a gene for replication control in pSAM2, the integrating element of Streptomyces ambofaciens. Mol Microbiol. 17(3):533-44. [PubMed:8559072]
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(6) Hagege J; Pernodet JL; Sezonov G; Gerbaud C; Friedmann A; Guerineau M (1993). Transfer functions of the conjugative integrating element pSAM2 from Streptomyces ambofaciens: characterization of a kil-kor system associated with transfer. J Bacteriol. 175(17):5529-38. [PubMed:8366038]
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(7) Hagege J; Boccard F; Smokvina T; Pernodet JL; Friedmann A; Guerineau M (1994). Identification of a gene encoding the replication initiator protein of the Streptomyces integrating element, pSAM2. Plasmid. 31(2):166-83. [PubMed:8029324]
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(8) Hagege J; Pernodet JL; Friedmann A; Guerineau M (1993). Mode and origin of replication of pSAM2, a conjugative integrating element of Streptomyces ambofaciens. Mol Microbiol. 10(4):799-812. [PubMed:7934842]
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(9) Pernodet JL; Simonet JM; Guerineau M (1984). Plasmids in different strains of Streptomyces ambofaciens: free and integrated form of plasmid pSAM2. Mol Gen Genet. 198(1):35-41. [PubMed:6596483]
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(10) Boccard F; Smokvina T; Pernodet JL; Friedmann A; Guerineau M (1989). The integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens is related to temperate bacteriophages. EMBO J. 8(3):973-80. [PubMed:2721504]
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(11) Boccard F; Smokvina T; Pernodet JL; Friedmann A; Guerineau M (1989). Structural analysis of loci involved in pSAM2 site-specific integration in Streptomyces. Plasmid. 21(1):59-70. [PubMed:2657820]
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(12) Kuhstoss S; Richardson MA; Rao RN (1989). Site-specific integration in Streptomyces ambofaciens: localization of integration functions in S. ambofaciens plasmid pSAM2. J Bacteriol. 171(1):16-23. [PubMed:2536654]
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| experimental literature |
| in silico analysis literature |