SecReT4
T4SS ID160
StrainCoxiella burnetii Dugway 5J108-111
Repliconchromosome [Browse all T4SS(s) in this replicon]
AccessionNC_009727
Location328950..361357
NameDot/Icm
Functioneffector translocation
ClassificationType IVB; Type I
experimental Experimental investigation has been performed on this T4SS.

T4SS components
ComponentDotADotBDotCDotDIcmBIcmCIcmDIcmEIcmF
Number111111110
ComponentIcmGIcmHIcmJIcmKIcmLIcmMIcmNIcmOIcmP
Number101120111
ComponentIcmQIcmRIcmSIcmTIcmVIcmWIcmXLvgA
Number10111110

The information of T4SS components from NC_009727
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1CBUD_0339324366..325451 [+], 1086154706588hypothetical protein 
2CBUD_0340325492..326256 [+], 765154705728alpha-L-glycero-D-manno-heptose beta-1,4-glucosyltransferase 
3CBUD_0341326409..326528 [+], 120154707544hypothetical protein 
4CBUD_0342 (hldE)326536..328026 [+], 1491209363764bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase 
5CBUD_0342a328051..328233 [-], 183209363765virulence-associated protein C 
6CBUD_0343328434..328868 [-], 435154707348hypothetical protein 
7CBUD_0344 (icmX)328950..330080 [-], 1131154706876IcmX  IcmX
8CBUD_0345330096..330554 [-], 459154707222hypothetical membrane associated protein 
9CBUD_0346 (icmW)330551..331000 [-], 450154706758IcmW  IcmW
10CBUD_0347 (icmV)331190..331684 [+], 495209363766IcmV  IcmV
11CBUD_0348 (dotA)331681..334125 [+], 2445209363767DotA  DotA
12CBUD_0348a334180..334275 [+], 96209363768hypothetical protein 
13CBUD_0349334597..335931 [-], 1335154707359glycine betaine transporter 
14CBUD_0350 (dotB)335938..337056 [-], 1119154706837ATP-binding protein  DotB
15CBUD_0351 (dotC)337094..337918 [-], 825154706650DotC  DotC
16CBUD_0352 (dotD)337921..338430 [-], 510154707117DotD  DotD
17CBUD_0353 (icmS)338587..338925 [-], 339154706376IcmS  IcmSaccesspry protein
18CBUD_0354 (icmT)338925..339182 [-], 258154706173IcmT  IcmT
19CBUD_0354a339193..339357 [-], 165209363769hypothetical protein 
20CBUD_0354b339527..340393 [+], 867209363770transposase 
21CBUD_0356340390..340662 [-], 273154707498hypothetical protein 
22CBUD_0358340890..342086 [+], 1197154706613hypothetical protein 
23CBUD_0359342198..343403 [+], 1206154705908ATPase 
24CBUD_0360343500..343637 [-], 138154707527hypothetical protein 
25CBUD_0361a345003..345176 [-], 174209363771hypothetical protein 
26CBUD_0363345292..345441 [+], 150154705871hypothetical protein 
27CBUD_0364 (icmQ)345438..346154 [+], 717154707715IcmQ  IcmQ
28CBUD_0365 (icmP)346155..347318 [+], 1164154706791IcmP  IcmP
29CBUD_0366 (icmO)347294..349672 [+], 2379154706520IcmO  IcmO
30CBUD_0367 (icmN)349682..350320 [+], 639209363772IcmN protein, OmpA family  IcmN
31CBUD_0368 (icmL2)350327..350950 [+], 624154705859IcmL  IcmL
32CBUD_0369 (icmL1)350952..351608 [+], 657154706496IcmL  IcmL
33CBUD_0370 (icmK)351619..352674 [+], 1056209363773IcmK  IcmK
34CBUD_0371 (icmE)352661..355780 [+], 3120209363774IcmE  IcmE
35CBUD_0372 (icmG)355798..356532 [+], 735154707284IcmG  IcmG
36CBUD_0373 (icmC)356603..357112 [+], 510154706548IcmC  IcmC
37CBUD_0374 (icmD)357206..357622 [+], 417154706258IcmD  IcmD
38CBUD_0375 (icmJ)357635..358273 [+], 639209363775IcmJ  IcmJ
39CBUD_0376 (icmB)358346..361357 [+], 3012154707026IcmB  IcmB
40CBUD_0377361372..361509 [-], 138154706252hypothetical protein 
41CBUD_0378361727..362398 [-], 672209363776hypothetical protein 
42CBUD_0378a362329..362682 [-], 354209363777hypothetical protein 
43CBUD_0378b362810..362944 [+], 135209363778hypothetical protein 
44CBUD_0379362973..364190 [+], 1218154707198hypothetical protein 
45CBUD_0381a364760..364915 [+], 156209363779hypothetical protein 
46CBUD_0381364828..365088 [-], 261154706208hypothetical protein 
 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

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Proteins        Genes

Effectors
CpeJ, CBUD_A0049, CpeI, CpeL, CBUD_0015, CBUD_0334, CBUD_0250, CBUD_0445, AnkG, CBUD_1786, AnkA, CBUD_2061, CBUD_2174, CBUD_0231, CBUD_2029, CBUD_1648, CBUD_2151, CBUD_1824, AnkM, CBUD_0430, CBUD_1204, CBUD_0821, CBUD_0997, CBUD_0461, CBUD_2108, CBUD_0422, CBUD_0454, CBUD_1994, CBUD_0648, CBUD_1925, CBUD_2172, CBUD_1674, CBUD_0137, CBUD_0236, CBUD_0496, CBUD_0358, CBUD_1609, CBUD_0462, CBUD_0561, CBUD_0395, CBUD_1894, CBUD_2113, CBUD_0572, CBUD_1474, CBUD_0053, CBUD_1562, CBUD_0675, CBUD_1910, CBUD_0410, CBUD_2147, CBUD_1664, CBUD_0444, AnkI, CBU_D1181, CBUD_2154, CBUD_2129, CBUD_2063, AnkI, CBUD_0886, CBUD_1711, CBUD_0646, AnkF, CBUD_0868, CBUD_0247, CBUD_0324, CBUD_0383, CBUD_1247, CpeK, CBUD_0054, CBUD_0053a, CBUD_0136, CBUD_0223a, AnkM, CBUD_0354b, CBUD_0378, CBUD_0419, CBUD_0487, CBUD_0493a, CBUD_0567a, CBUD_0588, CBUD_0615, CBUD_0618, CBUD_0949, CBUD_0996a, AnkH, AnkO, CBUD_1137, CBUD_1210, CBUD_1287, AnkI, CBUD_1402, CBUD_1462d, CBUD_1656, CBUD_1664c, CBUD_1685a, CBUD_1704, CBUD_1734, CBUD_1787, ElpA, AnkP, CBUD_2117, CBUD_2154a

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1CBUD_A0014 7840..8298 [+], 459154688257hypothetical proteinexperimental  CpeJ
2CBUD_A0049 36357..37109 [-], 753154688280hypothetical proteinexperimental  CBUD_A0049
3CBUD_A0009 3617..4513 [-], 897154688308hypothetical proteinexperimental  CpeI
4CBUD_A0024 15335..16189 [+], 855154688310tetratricopeptide repeat family proteinexperimental  CpeL
5CBUD_0015 18347..19213 [-], 867154705750gluconolactonaseexperimental  CBUD_0015
6CBUD_0334 318308..320047 [+], 1740154705759hypothetical proteinexperimental  CBUD_0334
7CBUD_0250 236844..238106 [-], 1263154705816hypothetical protein  CBUD_0250
8CBUD_0445 427863..428006 [-], 144154705839hypothetical protein  CBUD_0445
9CBUD_0829 805721..806737 [+], 1017154705900ankyrin repeat proteinexperimental  AnkGinteraction
10CBUD_1786 (algC)1782879..1784294 [-], 1416154705974phosphoglucomutase  CBUD_1786
11CBUD_2034 2009107..2010513 [-], 1407154706008ankyrin repeat proteinexperimental  AnkA
12CBUD_2061 2035716..2037848 [+], 2133154706084hypothetical protein  CBUD_2061
13CBUD_2174 2143039..2144085 [-], 1047154706085Fic family protein  CBUD_2174
14CBUD_0231 214610..215533 [-], 924154706141hypothetical protein  CBUD_0231
15CBUD_2029 2003553..2004344 [-], 792154706143hypothetical membrane spanning protein  CBUD_2029
16CBUD_1648 1643140..1644561 [+], 1422154706161hypothetical protein  CBUD_1648
17CBUD_2151 2123121..2123789 [-], 669154706197hypothetical protein  CBUD_2151
18CBUD_1824 1814396..1815616 [-], 1221154706230trans-2-enoyl-CoA reductase  CBUD_1824
19CBUD_0245 230535..233090 [+], 2556154706246ankyrin repeat proteinexperimental  AnkM
20CBUD_0430 412089..413792 [-], 1704154706247hypothetical membrane spanning protein  CBUD_0430
21CBUD_1204 1177390..1178436 [+], 1047154706250hypothetical cytosolic proteinexperimental  CBUD_1204
22CBUD_0821 (phnB)800870..801340 [+], 471154706267PhnBexperimental  CBUD_0821
23CBUD_0997 981158..982906 [-], 1749154706280hypothetical protein  CBUD_0997
24CBUD_0461 443861..446893 [+], 3033154706299hypothetical membrane associated protein  CBUD_0461
25CBUD_2108 2073430..2074617 [-], 1188154706300hypothetical proteinexperimental  CBUD_2108
26CBUD_0422 404434..405168 [-], 735154706307hypothetical proteinexperimental  CBUD_0422
27CBUD_0454 436483..437814 [-], 1332154706315hypothetical membrane spanning protein  CBUD_0454
28CBUD_1994 1970005..1970217 [+], 213154706316hypothetical proteinexperimental  CBUD_1994
29CBUD_0648 634460..635221 [-], 762154706334coenzyme PQQ synthesis protein Cexperimental  CBUD_0648
30CBUD_1925 (pkn2)1902717..1903457 [-], 741154706348serine/threonine protein kinase domain protein  CBUD_1925
31CBUD_2172 2141780..2142097 [+], 318154706420hypothetical proteinexperimental  CBUD_2172
32CBUD_1674 1666435..1668264 [+], 1830154706554hypothetical proteinexperimental  CBUD_1674
33CBUD_0137 122978..124231 [-], 1254154706571low-affinity inorganic phosphate transporter  CBUD_0137
34CBUD_0236 220470..221087 [+], 618154706600alpha/beta hydrolase  CBUD_0236
35CBUD_0496 477564..479411 [-], 1848154706607tetratricopeptide repeat family protein  CBUD_0496
36CBUD_0358 340890..342086 [+], 1197154706613hypothetical proteinexperimental  CBUD_0358
37CBUD_1609 1610704..1610907 [+], 204154706720hypothetical proteinexperimental  CBUD_1609
38CBUD_0462 446947..447942 [-], 996154706886hypothetical protein  CBUD_0462
39CBUD_0561 538277..538738 [+], 462154706898hypothetical proteinexperimental  CBUD_0561
40CBUD_0395 381225..381674 [-], 450154706917GatB/Yqey domain proteinexperimental  CBUD_0395
41CBUD_1894 1870217..1870612 [+], 396154706941ankyrin repeat proteinexperimental  CBUD_1894
42CBUD_2113 2080364..2081461 [-], 1098154706963hypothetical proteinexperimental  CBUD_2113
43CBUD_0572 552925..553377 [+], 45315470714717 kDa common-antigen  CBUD_0572
44CBUD_1474 1469186..1469359 [-], 174154707174hypothetical proteinexperimental  CBUD_1474
45CBUD_0053 51826..52875 [+], 1050154707221hypothetical protein  CBUD_0053
46CBUD_1562 1567708..1568856 [-], 1149154707234fructose-1,6-bisphosphataseexperimental  CBUD_1562
47CBUD_0675 655659..656738 [+], 1080154707282hypothetical proteinexperimental  CBUD_0675
48CBUD_1910 1887751..1888071 [-], 321154707301hypothetical proteinexperimental  CBUD_1910
49CBUD_0410 393661..396180 [-], 2520154707331hypothetical membrane spanning proteinexperimental  CBUD_0410
50CBUD_2147 2118917..2119813 [-], 897154707375hypothetical protein  CBUD_2147
51CBUD_1664 1656195..1657931 [+], 1737154707476hypothetical membrane spanning protein  CBUD_1664
52CBUD_0444 427164..427757 [+], 594154707518hypothetical protein  CBUD_0444
53CBUD_1298 1288705..1290885 [+], 2181154707569ankyrin repeat proteinexperimental  AnkI
54CBUD_1181 1153653..1154249 [-], 597154707571hypothetical proteinexperimental  CBU_D1181
55CBUD_2154 2126875..2128467 [-], 1593154707572hypothetical cytosolic proteinexperimental  CBUD_2154
56CBUD_2129 2096014..2096811 [-], 798154707574hypothetical proteinexperimental  CBUD_2129
57CBUD_2063 2038037..2040151 [+], 2115154707578hypothetical cytosolic protein  CBUD_2063
58CBUD_1305 1297411..1298004 [-], 594154707593hypothetical membrane spanning protein  AnkI
59CBUD_0886 871322..872653 [+], 1332154707605leucine-rich repeat proteinexperimental  CBUD_0886
60CBUD_1711 1707660..1709024 [+], 1365154707621fic family proteinexperimental  CBUD_1711
61CBUD_0646 632015..633505 [+], 1491154707634hypothetical membrane spanning protein  CBUD_0646
62CBUD_1627 1627582..1628136 [+], 555154707650ankyrin repeat proteinexperimental  AnkF
63CBUD_0868 (rimI)850598..851035 [+], 438154707659ribosomal-protein-S18-alanine acetyltransferase  CBUD_0868
64CBUD_0247 234462..235058 [+], 597154707693hypothetical proteinexperimental  CBUD_0247
65CBUD_0324 309202..309636 [+], 435154707731hypothetical cytosolic proteinexperimental  CBUD_0324
66CBUD_0383 368308..368706 [+], 399154707734hypothetical proteinexperimental  CBUD_0383
67CBUD_1247 1227849..1228508 [+], 660154707765hypothetical proteinexperimental  CBUD_1247
68CBUD_A0023 14915..15304 [+], 390209363627hypothetical proteinexperimental  CpeK
69CBUD_0054 53105..53524 [+], 420209363655hypothetical protein  CBUD_0054
70CBUD_0053a 52884..53114 [-], 231209363656hypothetical protein  CBUD_0053a
71CBUD_0136 121901..122713 [-], 813209363688hypothetical cytosolic proteinexperimental  CBUD_0136
72CBUD_0223a 204451..205317 [-], 867209363721transposase  CBUD_0223a
73CBUD_0245c 234327..234452 [+], 126209363733hypothetical protein  AnkM
74CBUD_0354b 339527..340393 [+], 867209363770transposase  CBUD_0354b
75CBUD_0378 361727..362398 [-], 672209363776hypothetical proteinexperimental  CBUD_0378
76CBUD_0419 401497..403140 [-], 1644209363786hypothetical cytosolic protein  CBUD_0419
77CBUD_0487 468473..470842 [+], 2370209363806hypothetical proteinexperimental  CBUD_0487
78CBUD_0493a 475600..476466 [-], 867209363808transposase  CBUD_0493a
79CBUD_0567a 544833..545699 [-], 867209363828transposase  CBUD_0567a
80CBUD_0588 567177..568010 [+], 834209363837hypothetical protein  CBUD_0588
81CBUD_0615 595246..596130 [-], 885209363844hypothetical protein  CBUD_0615
82CBUD_0618 599404..600576 [+], 1173209363846hypothetical proteinexperimental  CBUD_0618
83CBUD_0949 938311..939477 [+], 1167209363930hypothetical cytosolic proteinexperimental  CBUD_0949
84CBUD_0996a 980975..981088 [+], 114209363940hypothetical protein  CBUD_0996a
85CBUD_1019a 1005717..1006022 [-], 306209363947hypothetical protein  AnkH
86CBUD_1108 1086765..1088987 [+], 2223209363965ankyrin repeat proteinexperimental  AnkO
87CBUD_1137 1110238..1111689 [-], 1452209363975hypothetical protein  CBUD_1137
88CBUD_1210 1186511..1189687 [-], 3177209363998hypothetical membrane associated protein  CBUD_1210
89CBUD_1287 1273712..1274248 [+], 537209364027hypothetical cytosolic proteinexperimental  CBUD_1287
90CBUD_1298a 1290765..1290902 [+], 138209364033hypothetical cytosolic protein  AnkI
91CBUD_1402 1396642..1397286 [-], 645209364063hypothetical cytosolic protein  CBUD_1402
92CBUD_1462d 1460207..1460341 [+], 135209364082hypothetical protein  CBUD_1462d
93CBUD_1656 1651417..1652667 [-], 1251209364140hypothetical cytosolic protein  CBUD_1656
94CBUD_1664c 1658862..1658963 [+], 102209364143hypothetical protein  CBUD_1664c
95CBUD_1685a 1676512..1677378 [-], 867209364147transposase  CBUD_1685a
96CBUD_1704 1702443..1703165 [-], 723209364152hypothetical proteinexperimental  CBUD_1704
97CBUD_1734 1732275..1733063 [-], 789209364161hypothetical proteinexperimental  CBUD_1734
98CBUD_1787 1781413..1782882 [+], 1470209364177hypothetical protein  CBUD_1787
99CBUD_1884 1860679..1861935 [-], 1257209364199hypothetical membrane spanning proteinexperimental  ElpA
100CBUD_2035 2010501..2012543 [-], 2043209364250ankyrin repeat proteinexperimental  AnkP
101CBUD_2117 2083849..2084445 [+], 597209364285hypothetical cytosolic proteinexperimental  CBUD_2117
102CBUD_2154a 2128605..2128742 [-], 138209364298hypothetical protein  CBUD_2154a

interaction This effector contains information of interaction.

Download FASTA format files
Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1IcmS (chaperone) (154706376)NDAnkB, AnkH, AnkI, AnkJ, AnkMThe chaperone IcmS is essential for translocation into the host cytosol.(1) PubMed: 19411324
Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Voth DE; Howe D; Beare PA; Vogel JP; Unsworth N; Samuel JE; Heinzen RA (2009). The Coxiella burnetii ankyrin repeat domain-containing protein family is heterogeneous, with C-terminal truncations that influence Dot/Icm-mediated secretion. J Bacteriol. 191(13):4232-42. [PudMed:19411324] experimental
The information on host-pathogen interaction.

#Name(Protein GI)Host site/SubstrateSourceFunctionReference
1AnkG (154705900)gC1qR (p32)human, murineAnkG interacts with gC1qR(p32) to regulate an anti-apoptotic pathway which is required for adaptation to mammalian hosts of Coxiella.(1) PubMed: 20944063insolico

insolico This T4SE is highly identical with the protein mentioned in the literature.
(1) Luhrmann A; Nogueira CV; Carey KL; Roy CR (2010). Inhibition of pathogen-induced apoptosis by a Coxiella burnetii type IV effector protein. Proc Natl Acad Sci U S A. 107(44):18997-9001. [PudMed:20944063] experimental
(1) McDonough JA; Newton HJ; Roy CR (2012). Coxiella burnetii Secretion Systems. Adv Exp Med Biol. 984:171-97. [PudMed:22711632]
(2) Chen C; Banga S; Mertens K; Weber MM; Gorbaslieva I; Tan Y; Luo ZQ; Samuel JE (2010). Large-scale identification and translocation of type IV secretion substrates by Coxiella burnetii. Proc Natl Acad Sci U S A. 107(50):21755-60. [PudMed:21098666] experimental
(3) Voth DE; Howe D; Beare PA; Vogel JP; Unsworth N; Samuel JE; Heinzen RA (2009). The Coxiella burnetii ankyrin repeat domain-containing protein family is heterogeneous, with C-terminal truncations that influence Dot/Icm-mediated secretion. J Bacteriol. 191(13):4232-42. [PudMed:19411324] experimental
(4) Pan X; Luhrmann A; Satoh A; Laskowski-Arce MA; Roy CR (2008). Ankyrin repeat proteins comprise a diverse family of bacterial type IV effectors. Science. 320(5883):1651-4. [PudMed:18566289] experimental in_silico
 
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation

Genomics & Bioinformatics Group in MML, SJTU