oriTDB

The information of the oriT region

oriTDB accession number	200079
Name	oriT_MGIVchHai6
Sequence Completeness	intact
oriT length	49 nt
oriT sequence	TCCAAGATCCCCCATCAGATAGTTATTGGAAAGGAATTGGTAGGGTCTT
IR (inverted repeat)	_
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	49 bp minimal oriT
	D:

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCCAAGATCCCCCATCAGATAGTTATTGGAAAGGAATTGGTAGGGTCTT

Reference

[1] Carraro N et al (2016) IncA/C Conjugative Plasmids Mobilize a New Family of Multidrug Resistance Islands in Clinical Vibrio cholerae Non-O1/Non-O139 Isolates from Haiti. mBio. 7(4):e00509-16. [PMID:27435459]

[2] Rivard N et al (2020) Antibiotic Resistance in Vibrio cholerae: Mechanistic Insights from IncC Plasmid-Mediated Dissemination of a Novel Family of Genomic Islands Inserted at trmE. mSphere. 5(4):e00748-20. [PMID:32848007]

I. Information of Relaxase

ID	1068
Name	MobI_MGIVchHai6
GenBank accession number	ERP70969
Family	Other
Length	264 aa
UniProt ID	_
PDB ID	_
Pfam	MobI [PF19456.2], Evalue: 1E-34, Aligned region: 1..264
Note	putative relaxase
Protein sequence [Download]	MAKASQQKTRVPQKGTKAGDELTHETIDSLCIQHTVDYVMTKNYSFLEQQIVAFGAALNR GNQPQSEYIEAFSKSFKGLETQINIWETFIANSTNVNQDDAMLFSLSVSEQYLRYTHKSI EIEQIRYYYIAQKIADLFWQHNREHRGKNSPGYPGHFGCRVRLRNKKLEIFWVYNQFKPK KNSDGFQVISHYLPRDGHFRYPKTTFTRAQDWEKPVIEVVEDAFAIIRRTNANLMRIRQL CRWNNDNLYKMAGEIDDFKMDNPL

Reference

[1] Rivard N et al (2020) Antibiotic Resistance in Vibrio cholerae: Mechanistic Insights from IncC Plasmid-Mediated Dissemination of a Novel Family of Genomic Islands Inserted at trmE. mSphere. 5(4):e00748-20. [PMID:32848007]

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of mobile element

ID	87
Element type	IME
ICE description	MGIVchHai6
ICEberg accesion number	172_IME
GenBank accession number	AXDR01000001
Family
Genome size	47437
Coordinate of oriT [+/-]	43098..91582
Drug resistance	Antibiotic resistance genes: sul1 floR, tetA(G), tetR, floR
Heavy-metal resistance	Heavy-metal resistance genes: merR, merP, merA
Virulence factor	Virulence genes: RhuM
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Vibrio cholera non-01/non-0139 HC-36A1 [156539]

Reference

[1] Rivard N et al (2020) Antibiotic Resistance in Vibrio cholerae: Mechanistic Insights from IncC Plasmid-Mediated Dissemination of a Novel Family of Genomic Islands Inserted at trmE. mSphere. 5(4):e00748-20. [PMID:32848007]

[2] Carraro N et al (2016) IncA/C Conjugative Plasmids Mobilize a New Family of Multidrug Resistance Islands in Clinical Vibrio cholerae Non-O1/Non-O139 Isolates from Haiti. mBio. 7(4):e00509-16. [PMID:27435459]