oriTDB

The information of the oriT region

oriTDB accession number	200057
Name	oriT_R391
Sequence Completeness	intact
oriT length	299 nt
oriT sequence	GCTCTGTTTGGCGGTGGATGACTGAGCCAAAAAAATCGAGACGCCAAACGTCGTTTGCAT TCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTCAGGCATTT ATATGTGAAATCGGGCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATC GAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCA AACGGATAGTAGTTTTGGTTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
IR (inverted repeat)	[178-192] [216-230] nt (ATCGAGACGCCAAAC..GTTTGGCGTTTCGAT)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	_
	L:178-192;R:216-230

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GCTCTGTTTGGCGGTGGATGACTGAGCCAAAAAAATCGAGACGCCAAACGTCGTTTGCAT

61

TCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTCAGGCATTT

121

ATATGTGAAATCGGGCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATC

181

GAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCA

241

AACGGATAGTAGTTTTGGTTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA

Reference

[1] Ceccarelli D et al (2008) Identification of the origin of transfer (oriT) and a new gene required for mobilization of the SXT/R391 family of integrating conjugative elements. J Bacteriol. 190(15):5328-38. [PMID:18539733]

[2] Wozniak RA et al (2009) Comparative ICE genomics: insights into the evolution of the SXT/R391 family of ICEs. PLoS Genet. 5(12):e1000786. [PMID:20041216]

I. Information of Relaxase

ID	271
Name	TraI_R391
GenBank accession number	AAM08003
Family	MOB_H
Length	716 aa
UniProt ID	Q8RL15
PDB ID	_
Pfam	TraI_2 [PF07514.10], Evalue: 1.50E-121, Aligned region: 32..355
Note	_
Protein sequence [Download]	MFKNLFFQTKALPELSSQLDADIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQ RDFQRLIQPCIDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGT PVEKKELEPRWRVAAALGGLFHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIER YFIRWRDGRCKRHEQFSILVLNRVMTPELLAWLTQPGPEILQAMLEAIGNTDLEHVLSKL VIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRLLASSQWLVNQRDARVWLRKS NQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSASNERYESLAP EVLIKDDKPIWLPMLHISEADLLFSSNVPSSVRLFSKSEWEATRQTQAEPQSRSSEHSDL PEASSSIEHRKSTESPSTNSSEQDDELRLASDVNNPQATENAPGDGCEKPNNSYDGAISN NVNQYDTEALNLPESLAWLPEASSALVMVGEQILIRYPDAVRPWCAPRKLLAELSQLDWL ELDPANPTRKARTVTTNDGVQEQGLLLKVSISKRLIALIDISKQGTEPAAAIQNEEALQR PSRTKTTNAQAKEPATRAERKQKPIAPNAKSSTDPKYVQRQQMVNFVKDLPILLTDGDYP DVDHSADGIRVTIQTLRQVANAHGIPAGQLLRGISASDECQFDEGETVLFTAHAKR

Reference

[1] Ceccarelli D et al (2008) Identification of the origin of transfer (oriT) and a new gene required for mobilization of the SXT/R391 family of integrating conjugative elements. J Bacteriol. 190(15):5328-38. [PMID:18539733]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	360	_

Information of T4CP

ID	271
Name	TraD_R391
SecReT4 accesion number	_
GenBank accession number	AAM08004
Family	TrwB/TraD
Length	606
UniProt ID	Q8RL14
PDB ID	_
Pfam	TrwB_AAD_bind [PF10412.8], Evalue: 6.50E-10, Aligned region: 146..375 TrwB_AAD_bind [PF10412.8], Evalue: 1.40E-05, Aligned region: 406..592 TraG-D_C [PF12696.6], Evalue: 2.20E-12, Aligned region: 451..579 AAA_10 [PF12846.6], Evalue: 7.60E-08, Aligned region: 146..291 DUF87 [PF01935.16], Evalue: 4.60E-05, Aligned region: 152..231
Note	putative coupling protein involved in conjugal transfer
Protein sequence [Download]

Reference

[1] Ceccarelli D et al (2008) Comparative ICE genomics: insights into the evolution of the SXT/R391 family of ICEs. J Bacteriol. 190(15):5328-38. [PMID:18539733]

Information of mobile element

ID	65
Element type	Conjugative genomic island R391
ICE description	R391
ICEberg accesion number	43
GenBank accession number	AY090559.1
Family	SXT/R391
Genome size	88532 bp
Coordinate of oriT [+/-]	5428..5726 [+]
Drug resistance	kanamycin resistance
Heavy-metal resistance	mercury resistance
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Providencia rettgeri [587]

Reference

[1] Ceccarelli D et al (2008) Identification of the origin of transfer (oriT) and a new gene required for mobilization of the SXT/R391 family of integrating conjugative elements. J Bacteriol. 190(15):5328-38. [PMID:18539733]

[2] Peters SE et al (1991) Novel mercury resistance determinants carried by IncJ plasmids pMERPH and R391. Mol Gen Genet. 228(1-2):294-9. [PMID:1886614]