oriTDB
The information of the oriT region
oriTDB accession number 200057
Name oriT_R391  experimental
Sequence Completeness intact
oriT length 299 nt
oriT sequence GCTCTGTTTGGCGGTGGATGACTGAGCCAAAAAAATCGAGACGCCAAACGTCGTTTGCAT TCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTCAGGCATTT ATATGTGAAATCGGGCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATC GAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCA AACGGATAGTAGTTTTGGTTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
IR (inverted repeat)[178-192] [216-230] nt  (ATCGAGACGCCAAAC..GTTTGGCGTTTCGAT)
Location of nic site _
Conserved sequence flanking the
  nic site 		_
Note _
L:178-192;R:216-230
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Ceccarelli D et al (2008) Identification of the origin of transfer (oriT) and a new gene required for mobilization of the SXT/R391 family of integrating conjugative elements. J Bacteriol. 190(15):5328-38. [PMID:18539733]
[2] Wozniak RA et al (2009) Comparative ICE genomics: insights into the evolution of the SXT/R391 family of ICEs. PLoS Genet. 5(12):e1000786. [PMID:20041216]
I. Information of Relaxase
ID 271
Name TraI_R391 insolico
GenBank accession number AAM08003
Family MOBH
Length 716 aa
UniProt ID Q8RL15
PDB ID _
Pfam TraI_2 [PF07514.10], Evalue: 1.50E-121, Aligned region: 32..355
Note _
Protein sequence [Download] MFKNLFFQTKALPELSSQLDADIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQ
RDFQRLIQPCIDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGT
PVEKKELEPRWRVAAALGGLFHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIER
YFIRWRDGRCKRHEQFSILVLNRVMTPELLAWLTQPGPEILQAMLEAIGNTDLEHVLSKL
VIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRLLASSQWLVNQRDARVWLRKS
NQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSASNERYESLAP
EVLIKDDKPIWLPMLHISEADLLFSSNVPSSVRLFSKSEWEATRQTQAEPQSRSSEHSDL
PEASSSIEHRKSTESPSTNSSEQDDELRLASDVNNPQATENAPGDGCEKPNNSYDGAISN
NVNQYDTEALNLPESLAWLPEASSALVMVGEQILIRYPDAVRPWCAPRKLLAELSQLDWL
ELDPANPTRKARTVTTNDGVQEQGLLLKVSISKRLIALIDISKQGTEPAAAIQNEEALQR
PSRTKTTNAQAKEPATRAERKQKPIAPNAKSSTDPKYVQRQQMVNFVKDLPILLTDGDYP
DVDHSADGIRVTIQTLRQVANAHGIPAGQLLRGISASDECQFDEGETVLFTAHAKR
Reference
[1] Ceccarelli D et al (2008) Identification of the origin of transfer (oriT) and a new gene required for mobilization of the SXT/R391 family of integrating conjugative elements. J Bacteriol. 190(15):5328-38. [PMID:18539733]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 360 _ insolico
Information of T4CP
ID 271
Name TraD_R391 insolico
SecReT4 accesion number _
GenBank accession number AAM08004
Family TrwB/TraD
Length 606
UniProt ID Q8RL14
PDB ID _
Pfam TrwB_AAD_bind [PF10412.8], Evalue: 6.50E-10, Aligned region: 146..375
TrwB_AAD_bind [PF10412.8], Evalue: 1.40E-05, Aligned region: 406..592
TraG-D_C [PF12696.6], Evalue: 2.20E-12, Aligned region: 451..579
AAA_10 [PF12846.6], Evalue: 7.60E-08, Aligned region: 146..291
DUF87 [PF01935.16], Evalue: 4.60E-05, Aligned region: 152..231
Note putative coupling protein involved in conjugal transfer
Protein sequence [Download]

Reference
[1] Ceccarelli D et al (2008) Comparative ICE genomics: insights into the evolution of the SXT/R391 family of ICEs. J Bacteriol. 190(15):5328-38. [PMID:18539733]
Information of mobile element
ID 65
Element type Conjugative genomic island R391
ICE description R391
ICEberg accesion number 43
GenBank accession number AY090559.1
Family SXT/R391
Genome size 88532 bp
Coordinate of oriT  [+/-] 5428..5726 [+]
Drug resistance experimental kanamycin resistance
Heavy-metal resistance experimental mercury resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Providencia rettgeri [587]
Reference
[1] Ceccarelli D et al (2008) Identification of the origin of transfer (oriT) and a new gene required for mobilization of the SXT/R391 family of integrating conjugative elements. J Bacteriol. 190(15):5328-38. [PMID:18539733]
[2] Peters SE et al (1991) Novel mercury resistance determinants carried by IncJ plasmids pMERPH and R391. Mol Gen Genet. 228(1-2):294-9. [PMID:1886614]

Microbial Bioinformatics Group in MML, SJTU