 oriTDB accession number | 200056 |
| Name | oriT_SXT(MO10)  |
Sequence Completeness  | intact |
| oriT length | 299 nt |
| oriT sequence | GCTCTGTTTGGCGGCGGATGACCTAGTCAAAAAAATCGAGACGCCAAACGTCGTTTGCAT TCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGACGATACGTCGTTTTAGGCGTTT TTAAGTGAAATCGGGCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATC GAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCA AACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA |
| IR (inverted repeat) | [178-192] [216-230] nt (ATCGAGACGCCAAAC..GTTTGGCGTTTCGAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site | _ |
| Note | _ |
Visualization of oriT structure (The oriT was characterized experimentally) Reference| [1] Peters SE et al (1991) Novel mercury resistance determinants carried by IncJ plasmids pMERPH and R391. Mol Gen Genet. 228(1-2):294-9. [PMID:1886614] |
| [2] Wozniak RA et al (2009) Comparative ICE genomics: insights into the evolution of the SXT/R391 family of ICEs. PLoS Genet. 5(12):e1000786. [PMID:20041216] |
| [3] Daccord A et al (2010) Integrating conjugative elements of the SXT/R391 family trigger the excision and drive the mobilization of a new class of Vibrio genomic islands. Mol Microbiol. 78(3):576-88. [PMID:20807202] |
| ID | 270 |
| Name | TraI_SXT(MO10)  |
| GenBank accession number | AAL59675 |
| Family | MOBH |
| Length | 716 aa |
| UniProt ID | Q8KQY3 |
| PDB ID | _ |
| Pfam | TraI_2 [PF07514.10], Evalue: 3.10E-121, Aligned region: 32..355 |
| Note | conjugative relaxase |
| Protein sequence [Download] | MFKNLFFQAKALPDLSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNL RDFQRLIQPCIDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGT PVEKKELEPRWRVAAALGGLFHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIER YFIRWRDGRCKRHEQFSILVLNRVMTPELLAWLTQPGPEILQAMLEAIGNTDPEHVLSKL VIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRLLASSQWLVNQRDARVWLRKS NQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSASNERYESLAP EVLIKDDKPIWLPMLHISEADLLFSSNVPSSVRLFSKSEWEATQKTQEEPQSHSSEHSDL PEASSSIEHRKSDESPSTNSSEQENELRHASDVSNPQATENAPGDECEKPNDSSDADIAN NANQHHAEALNLPESLAWLPEASSALVMVGEQVLIRYPDAVRHWCAPRKLLAELSRLDWL ELDPANLTRKARTVTTNDGVQEQGLLLKVSISKGLTALIDLPKQDAEPAAAIQNEEALQR PSRTETTNAQAKEPATRAERKQKPIAANTNSSTDPKHAQRQQMVNFVKDLPILLTDGDYP DVDHSADGIRVTIQTLRQVANAHGIPAGQLLRGISASDQCQFDEGETVLFTAHAKR |
Reference| [1] Ceccarelli D et al (2008) Identification of the origin of transfer (oriT) and a new gene required for mobilization of the SXT/R391 family of integrating conjugative elements. J Bacteriol. 190(15):5328-38. [PMID:18539733] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 359 | _ |
| ID | 270 |
| Name | TraD_SXT(MO10) |
| SecReT4 accesion number | _ |
| GenBank accession number | AAL59680 |
| Family | TrwB/TraD |
| Length | 599 |
| UniProt ID | Q8KQY2 |
| PDB ID | _ |
| Pfam | TrwB_AAD_bind [PF10412.8], Evalue: 6.40E-10, Aligned region: 139..367 TrwB_AAD_bind [PF10412.8], Evalue: 1.30E-05, Aligned region: 399..585 TraG-D_C [PF12696.6], Evalue: 2.60E-12, Aligned region: 444..572 AAA_10 [PF12846.6], Evalue: 7.50E-08, Aligned region: 139..284 DUF87 [PF01935.16], Evalue: 4.50E-05, Aligned region: 145..224 |
| Note | _ |
| Protein sequence [Download] |
Reference| [1] Ceccarelli D et al (2008) Integrating conjugative elements of the SXT/R391 family trigger the excision and drive the mobilization of a new class of Vibrio genomic islands. J Bacteriol. 190(15):5328-38. [PMID:18539733] |
| ID | 64 |
| Element type | contains SXT transposon-like element |
| ICE description | SXT(MO10) |
| ICEberg accesion number | 44 |
| GenBank accession number | AY055428.1 |
| Family | SXT/R391 |
| Genome size | 99483 bp |
| Coordinate of oriT [+/-] | 3516..3814 [+] |
| Drug resistance | resistance to trimethoprim, sulfamethoxazole, streptomycin, and furazolidone |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Vibrio cholerae O139 MO10 [666] |
Reference| [1] Ceccarelli D et al (2008) Identification of the origin of transfer (oriT) and a new gene required for mobilization of the SXT/R391 family of integrating conjugative elements. J Bacteriol. 190(15):5328-38. [PMID:18539733] |
| [2] Waldor MK et al (1996) A new type of conjugative transposon encodes resistance to sulfamethoxazole, trimethoprim, and streptomycin in Vibrio cholerae O139. J Bacteriol. 178(14):4157-65. [PMID:8763944] |
| [3] Daccord A et al (2010) Integrating conjugative elements of the SXT/R391 family trigger the excision and drive the mobilization of a new class of Vibrio genomic islands. Mol Microbiol. 78(3):576-88. [PMID:20807202] |