oriTDB

The information of the oriT region

oriTDB accession number	200012
Name	oriT_ ICEEc1
Sequence Completeness	intact
oriT length	250 nt
oriT sequence	GCACACCGTCGCAGCCAGGCTATATTTCCCGCCCCGGCGGGGAATATGCCGATTAGGCGC GACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTA AACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGACGACGGTGTGCCGCCTTTGACCC TGGGGGTTTTGGCGTGGCGTCCAGCCGCGACATTACCCCCATGCACCGGGCAATAAGGGC GTCCAGCCCG
IR (inverted repeat)	[1-13] [156-168] nt (GCACACCGTCGCA..TGCGACGGTGTGC) [57-75] [131-149] nt (GCGCGACCAACCCCTTTAA..TTAAAGGGGTTGGTCGCGC)
Location of nic site	[142-143] nt
Conserved sequence flanking the nic site	GGTTG\|GTCGCG
Note	_
	L:1-13;R:156-168;L:57-75;R:131-149;D:142-143

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GCACACCGTCGCAGCCAGGCTATATTTCCCGCCCCGGCGGGGAATATGCCGATTAGGCGC

61

GACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTA

121

AACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGACGACGGTGTGCCGCCTTTGACCC

181

TGGGGGTTTTGGCGTGGCGTCCAGCCGCGACATTACCCCCATGCACCGGGCAATAAGGGC

241

GTCCAGCCCG

Reference

[1] Lin TL et al (2008) Characterization of integrative and conjugative element ICEKp1-associated genomic heterogeneity in a Klebsiella pneumoniae strain isolated from a primary liver abscess. J Bacteriol. 190(2):515-26. [PMID:17981959]

[2] Marcoleta AE et al (2016) Klebsiella pneumoniae Asparagine tDNAs Are Integration Hotspots for Different Genomic Islands Encoding Microcin E492 Production Determinants and Other Putative Virulence Factors Present in Hypervirulent Strains. Front Microbiol. 0.88125. [PMID:27375573]

N/A

Information of mobile element

ID	17
Element type	ICE (Conjugative transposon);high-pathogenicity island
ICE description	ICEEc1
ICEberg accesion number	70
GenBank accession number	AY233333.1
Family	ICEKp1
Genome size	40365 bp
Coordinate of oriT [+/-]	17654..17903 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	high-pathogenicity island
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli ECOR31 [562]

Reference

[1] Schubert S et al (2004) A novel integrative and conjugative element (ICE) of Escherichia coli: the putative progenitor of the Yersinia high-pathogenicity island. Mol Microbiol. 51(3):837-48. [PMID:14731283]

[2] Lin TL et al (2008) Characterization of integrative and conjugative element ICEKp1-associated genomic heterogeneity in a Klebsiella pneumoniae strain isolated from a primary liver abscess. J Bacteriol. 190(2):515-26. [PMID:17981959]

[3] Marcoleta AE et al (2016) Klebsiella pneumoniae Asparagine tDNAs Are Integration Hotspots for Different Genomic Islands Encoding Microcin E492 Production Determinants and Other Putative Virulence Factors Present in Hypervirulent Strains. Front Microbiol. 0.88125. [PMID:27375573]