oriTDB
The information of the oriT region
oriTDB accession number 200011
Name oriT_NBU2 (IME)  insolico
Sequence Completeness intact
oriT length 219 nt
oriT sequence TCCGCATGGAATACGGCAGAGAGCGGTACATCCGGGACGCTTCGCAGATTTACAGAGGAT GCAAGGACTTGAACGAATACTTACAGAAACAGATTGAAAGAAAAAGGCAGCTCCAGTCCG CCAAAGGGGTGCGCAGCCAATCACCGGAAAAGAAGAACGGCTTCCAGTTATAGCCCACTA TAACTACACGCTTCGCTTTCGTAGTTGTGGGCTCTCCCGGGG
IR (inverted repeat)[166-173] [178-185] nt  (AGTTATAG..CTATAACT)
Location of nic site _
Conserved sequence flanking the
  nic site 		_
Note similar to NBU1 oriT region
L:166-173;R:178-185
Visualization of oriT structure
Reference
[1] Wang J et al (2000) Characterization of a Bacteroides mobilizable transposon, NBU2, which carries a functional lincomycin resistance gene. J Bacteriol. 182(12):3559-71. [PMID:10852890]
[2] Li LY et al (1995) The mobilization regions of two integrated Bacteroides elements, NBU1 and NBU2, have only a single mobilization protein and may be on a cassette. J Bacteriol. 177(14):3940-5. [PMID:7608064]
I. Information of Relaxase
ID 225
Name Mob_NBU2 insolico
GenBank accession number AAA87350
Family MOBP
Length 467 aa
UniProt ID Q45639
PDB ID _
Pfam
Note _
Protein sequence [Download] MATKSSIHIKPCNIASSEAHNRRTAEYMRNIGESRIYVVPELSTDNEQWINPDFGTPELR
THYDNIKQMVKEKTGRAMQEKERERKGKNGKIIKVAGCSPIREGVLLIRPDTTLADVRKF
GEECQRRWGITPLQIFLHKDEGHWLNGQPEAEDKESFQVGNRWFKPNYHAHVVFDWMNHE
TGKSRKLNDEDMATMQTLASDILLMERGQAKAVTGKEHLERNDFIIEKQKAELQRIEETK
RHKEQQVSLAEQELKQVKAEIRTDKLKKTATNAATAIASGVGSLFGSGKLKELEHHIEQL
HQEITKRDKATDELKIQIQQIQEQHSKQIRNLQRIHNQELEAKDKEISRLSTLLEKAHKW
FPMFKEMLRMEKLCAVIGFTKDMIENLLTKKESIQCSGKIYSEEHRWKFDIKNDIFRLEK
HPMDSWKLMLTINRQPIVQWFKEQWEKLQQNLRNSVQREQKSRGFRI
Reference
[1] Li LY et al (1995) The mobilization regions of two integrated Bacteroides elements, NBU1 and NBU2, have only a single mobilization protein and may be on a cassette. J Bacteriol. 177(14):3940-5. [PMID:7608064]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 313 _ insolico
 N/A
Information of mobile element
ID 16
Element type IME (Mobilizable transposon)
ICE description NBU2
ICEberg accesion number _
GenBank accession number L42370.1
Family _
Genome size _
Coordinate of oriT  [+/-]
Drug resistance experimental carries a functional lincomycin resistance gene
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Bacteroides sp. [29523]
Reference
[1] Wang J et al (2000) Characterization of a Bacteroides mobilizable transposon, NBU2, which carries a functional lincomycin resistance gene. J Bacteriol. 182(12):3559-71. [PMID:10852890]

Microbial Bioinformatics Group in MML, SJTU