oriTDB

The information of the oriT region

oriTDB accession number	200011
Name	oriT_NBU2 (IME)
Sequence Completeness	intact
oriT length	219 nt
oriT sequence	TCCGCATGGAATACGGCAGAGAGCGGTACATCCGGGACGCTTCGCAGATTTACAGAGGAT GCAAGGACTTGAACGAATACTTACAGAAACAGATTGAAAGAAAAAGGCAGCTCCAGTCCG CCAAAGGGGTGCGCAGCCAATCACCGGAAAAGAAGAACGGCTTCCAGTTATAGCCCACTA TAACTACACGCTTCGCTTTCGTAGTTGTGGGCTCTCCCGGGG
IR (inverted repeat)	[166-173] [178-185] nt (AGTTATAG..CTATAACT)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	similar to NBU1 oriT region
	L:166-173;R:178-185

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCCGCATGGAATACGGCAGAGAGCGGTACATCCGGGACGCTTCGCAGATTTACAGAGGAT

61

GCAAGGACTTGAACGAATACTTACAGAAACAGATTGAAAGAAAAAGGCAGCTCCAGTCCG

121

CCAAAGGGGTGCGCAGCCAATCACCGGAAAAGAAGAACGGCTTCCAGTTATAGCCCACTA

181

TAACTACACGCTTCGCTTTCGTAGTTGTGGGCTCTCCCGGGG

Reference

[1] Wang J et al (2000) Characterization of a Bacteroides mobilizable transposon, NBU2, which carries a functional lincomycin resistance gene. J Bacteriol. 182(12):3559-71. [PMID:10852890]

[2] Li LY et al (1995) The mobilization regions of two integrated Bacteroides elements, NBU1 and NBU2, have only a single mobilization protein and may be on a cassette. J Bacteriol. 177(14):3940-5. [PMID:7608064]

I. Information of Relaxase

ID	225
Name	Mob_NBU2
GenBank accession number	AAA87350
Family	MOB_P
Length	467 aa
UniProt ID	Q45639
PDB ID	_
Pfam
Note	_
Protein sequence [Download]	MATKSSIHIKPCNIASSEAHNRRTAEYMRNIGESRIYVVPELSTDNEQWINPDFGTPELR THYDNIKQMVKEKTGRAMQEKERERKGKNGKIIKVAGCSPIREGVLLIRPDTTLADVRKF GEECQRRWGITPLQIFLHKDEGHWLNGQPEAEDKESFQVGNRWFKPNYHAHVVFDWMNHE TGKSRKLNDEDMATMQTLASDILLMERGQAKAVTGKEHLERNDFIIEKQKAELQRIEETK RHKEQQVSLAEQELKQVKAEIRTDKLKKTATNAATAIASGVGSLFGSGKLKELEHHIEQL HQEITKRDKATDELKIQIQQIQEQHSKQIRNLQRIHNQELEAKDKEISRLSTLLEKAHKW FPMFKEMLRMEKLCAVIGFTKDMIENLLTKKESIQCSGKIYSEEHRWKFDIKNDIFRLEK HPMDSWKLMLTINRQPIVQWFKEQWEKLQQNLRNSVQREQKSRGFRI

Reference

[1] Li LY et al (1995) The mobilization regions of two integrated Bacteroides elements, NBU1 and NBU2, have only a single mobilization protein and may be on a cassette. J Bacteriol. 177(14):3940-5. [PMID:7608064]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	313	_

N/A

Information of mobile element

ID	16
Element type	IME (Mobilizable transposon)
ICE description	NBU2
ICEberg accesion number	_
GenBank accession number	L42370.1
Family	_
Genome size	_
Coordinate of oriT [+/-]
Drug resistance	carries a functional lincomycin resistance gene
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bacteroides sp. [29523]

Reference

[1] Wang J et al (2000) Characterization of a Bacteroides mobilizable transposon, NBU2, which carries a functional lincomycin resistance gene. J Bacteriol. 182(12):3559-71. [PMID:10852890]