oriTDB
The information of the oriT region
oriTDB accession number 200008
Name oriT_Tn4555 (IME)  experimental
Sequence Completeness intact
oriT length 174 nt
oriT sequence CCCTCGGGAGAGCCCACAACTACGTAAGCGGAGCGTGTAGTTATAGTGGGCTATATCAAT GGCAAGCCATTGTCTGCAAACTCCAGCCTACGGCTTCCGCTCTCCTCCGTCAGGGAGGTT TTTCATCATCGTTGCCGATTGGAGATGCACCGACCAGCACAAGGTCTAAATCGT
IR (inverted repeat)[20-24] [29-33] nt  (CTACG..CGGAG)
[57-63] [66-72] nt  (CAATGGC..GCCATTG)
[104-110] [112-118] nt  (CCTCCGT..AGGGAGG)
Location of nic site [50-51] nt
Conserved sequence flanking the
  nic site 		_
Note Its oriT shows significant homology to the nic sites of the pMV158-oriT family.
L:20-24;R:29-33;L:57-63;R:66-72;L:104-110;R:112-118;D:50-51
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Tribble GD et al (1997) The Bacteroides mobilizable transposon Tn4555 integrates by a site-specific recombination mechanism similar to that of the gram-positive bacterial element Tn916. J Bacteriol. 179(8):2731-9. [PMID:9098073]
[2] Vedantam G et al (2006) Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation. Mol Microbiol. 59(1):288-300. [PMID:16359335]
[3] Smith CJ et al (1998) The transfer origin for Bacteroides mobilizable transposon Tn4555 is related to a plasmid family from gram-positive bacteria. J Bacteriol. 180(2):435-9. [PMID:9440538]
[4] Grohmann E et al (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]
[5] Grohmann E et al (1999) Mobilisation of the streptococcal plasmid pMV158: interactions of MobM protein with its cognate oriT DNA region. Mol Gen Genet. 261(4-5):707-15. [PMID:10394908]
I. Information of Relaxase
ID 222
Name MobA_Tn4555 insolico
GenBank accession number AAD43599
Family MOBV
Length 519 aa
UniProt ID Q9XBG8
PDB ID _
Pfam
Note relaxase
Protein sequence [Download] MTHSGRFSPENPEQRVTLSLQSPLMGCTPKNPMRLRTAEKQTIKYKPKKQVSMATKSSIH
IKPCNIASSEAHNRRTAEYMRHIGESRTYVVPELSTDNEQWINPDFGSPDLRMHYDNIRQ
MVKEKTGRAMQEKERERKGKNGKIVKIAGCSPIREGVLLVRSDTTLADVRKFGEECQRRW
GITPLQIFLHKDEGHWLNGQPEAEDRESFKVGDRWFKPNYHAHIVFDWMNHETGKSRKLN
DDDMMQMQTLASDILLMERGQSKAVTGKEHLERNDFIIEKQKAELQRMDAAKRHKEEQIN
LAEQELKQVKSEIRTDKLKKTATTAATAITSGVASLFGSGKLKELERANEKLQDEVSKRN
TNIEKLQSQVQQMSETAYTQIHNLREMHRQELDMKEKELSRLARIIDKAFRWFPMFREML
RMEKFCAMLGFSKEMTESLIVKKEALKCSGKIYSEQHRRNFDIKDDILRVENDPDDESRL
NLTINRKPIADWFREQWHRLRYGARVPQQEERKSRGFKL
Reference
[1] Smith CJ et al (1996) A gene product related to Tral is required for the mobilization of Bacteroides mobilizable transposons and plasmids. Mol Microbiol. 20(4):741-50. [PMID:8793871]
[2] Tribble GD et al (1997) The Bacteroides mobilizable transposon Tn4555 integrates by a site-specific recombination mechanism similar to that of the gram-positive bacterial element Tn916. J Bacteriol. 179(8):2731-9. [PMID:9098073]
[3] Smith CJ et al (1998) The transfer origin for Bacteroides mobilizable transposon Tn4555 is related to a plasmid family from gram-positive bacteria. J Bacteriol. 180(2):435-9. [PMID:9440538]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 310 _ insolico
 N/A
Information of mobile element
ID 13
Element type IME (Mobilizable transposon); beta-lactamase transposon Tn4555
ICE description Tn4555
ICEberg accesion number _
GenBank accession number U75371.3
Family _
Genome size 12105 bp
Coordinate of oriT  [+/-] 6390..6563 [+]
Drug resistance experimental resistance to cefoxitin (the beta-lactamase gene cfxA)
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Bacteroides fragilis [817]
Reference
[1] Tribble GD et al (1997) The Bacteroides mobilizable transposon Tn4555 integrates by a site-specific recombination mechanism similar to that of the gram-positive bacterial element Tn916. J Bacteriol. 179(8):2731-9. [PMID:9098073]

Microbial Bioinformatics Group in MML, SJTU