oriTDB

The information of the oriT region

oriTDB accession number	200008
Name	oriT_Tn4555 (IME)
Sequence Completeness	intact
oriT length	174 nt
oriT sequence	CCCTCGGGAGAGCCCACAACTACGTAAGCGGAGCGTGTAGTTATAGTGGGCTATATCAAT GGCAAGCCATTGTCTGCAAACTCCAGCCTACGGCTTCCGCTCTCCTCCGTCAGGGAGGTT TTTCATCATCGTTGCCGATTGGAGATGCACCGACCAGCACAAGGTCTAAATCGT
IR (inverted repeat)	[20-24] [29-33] nt (CTACG..CGGAG) [57-63] [66-72] nt (CAATGGC..GCCATTG) [104-110] [112-118] nt (CCTCCGT..AGGGAGG)
Location of nic site	[50-51] nt
Conserved sequence flanking the nic site	_
Note	Its oriT shows significant homology to the nic sites of the pMV158-oriT family.
	L:20-24;R:29-33;L:57-63;R:66-72;L:104-110;R:112-118;D:50-51

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCCTCGGGAGAGCCCACAACTACGTAAGCGGAGCGTGTAGTTATAGTGGGCTATATCAAT

61

GGCAAGCCATTGTCTGCAAACTCCAGCCTACGGCTTCCGCTCTCCTCCGTCAGGGAGGTT

121

TTTCATCATCGTTGCCGATTGGAGATGCACCGACCAGCACAAGGTCTAAATCGT

Reference

[1] Tribble GD et al (1997) The Bacteroides mobilizable transposon Tn4555 integrates by a site-specific recombination mechanism similar to that of the gram-positive bacterial element Tn916. J Bacteriol. 179(8):2731-9. [PMID:9098073]

[2] Vedantam G et al (2006) Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation. Mol Microbiol. 59(1):288-300. [PMID:16359335]

[3] Smith CJ et al (1998) The transfer origin for Bacteroides mobilizable transposon Tn4555 is related to a plasmid family from gram-positive bacteria. J Bacteriol. 180(2):435-9. [PMID:9440538]

[4] Grohmann E et al (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]

[5] Grohmann E et al (1999) Mobilisation of the streptococcal plasmid pMV158: interactions of MobM protein with its cognate oriT DNA region. Mol Gen Genet. 261(4-5):707-15. [PMID:10394908]

I. Information of Relaxase

ID	222
Name	MobA_Tn4555
GenBank accession number	AAD43599
Family	MOB_V
Length	519 aa
UniProt ID	Q9XBG8
PDB ID	_
Pfam
Note	relaxase
Protein sequence [Download]	MTHSGRFSPENPEQRVTLSLQSPLMGCTPKNPMRLRTAEKQTIKYKPKKQVSMATKSSIH IKPCNIASSEAHNRRTAEYMRHIGESRTYVVPELSTDNEQWINPDFGSPDLRMHYDNIRQ MVKEKTGRAMQEKERERKGKNGKIVKIAGCSPIREGVLLVRSDTTLADVRKFGEECQRRW GITPLQIFLHKDEGHWLNGQPEAEDRESFKVGDRWFKPNYHAHIVFDWMNHETGKSRKLN DDDMMQMQTLASDILLMERGQSKAVTGKEHLERNDFIIEKQKAELQRMDAAKRHKEEQIN LAEQELKQVKSEIRTDKLKKTATTAATAITSGVASLFGSGKLKELERANEKLQDEVSKRN TNIEKLQSQVQQMSETAYTQIHNLREMHRQELDMKEKELSRLARIIDKAFRWFPMFREML RMEKFCAMLGFSKEMTESLIVKKEALKCSGKIYSEQHRRNFDIKDDILRVENDPDDESRL NLTINRKPIADWFREQWHRLRYGARVPQQEERKSRGFKL

Reference

[1] Smith CJ et al (1996) A gene product related to Tral is required for the mobilization of Bacteroides mobilizable transposons and plasmids. Mol Microbiol. 20(4):741-50. [PMID:8793871]

[2] Tribble GD et al (1997) The Bacteroides mobilizable transposon Tn4555 integrates by a site-specific recombination mechanism similar to that of the gram-positive bacterial element Tn916. J Bacteriol. 179(8):2731-9. [PMID:9098073]

[3] Smith CJ et al (1998) The transfer origin for Bacteroides mobilizable transposon Tn4555 is related to a plasmid family from gram-positive bacteria. J Bacteriol. 180(2):435-9. [PMID:9440538]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	310	_

N/A

Information of mobile element

ID	13
Element type	IME (Mobilizable transposon); beta-lactamase transposon Tn4555
ICE description	Tn4555
ICEberg accesion number	_
GenBank accession number	U75371.3
Family	_
Genome size	12105 bp
Coordinate of oriT [+/-]	6390..6563 [+]
Drug resistance	resistance to cefoxitin (the beta-lactamase gene cfxA)
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bacteroides fragilis [817]

Reference

[1] Tribble GD et al (1997) The Bacteroides mobilizable transposon Tn4555 integrates by a site-specific recombination mechanism similar to that of the gram-positive bacterial element Tn916. J Bacteriol. 179(8):2731-9. [PMID:9098073]