oriTDB

The information of the oriT region

oriTDB accession number	200007
Name	oriT_Tn5520 (IME)
Sequence Completeness	intact
oriT length	71 nt
oriT sequence	CTTATTGGGGAATTTTCAGCGATACGGAGTATTGCGGCTCGGAAAATTCCCTAATAAGCT ACGGTATTTTC
IR (inverted repeat)	[14-30] [55-71] nt (CTTATTAGGGAATTTTC..GAAAATTCCCCAATAAG)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	71 bp minimal oriT
	L:14-30;R:55-71

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CTTATTGGGGAATTTTCAGCGATACGGAGTATTGCGGCTCGGAAAATTCCCTAATAAGCT

61

ACGGTATTTTC

Reference

[1] Vedantam G et al (2006) Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation. Mol Microbiol. 59(1):288-300. [PMID:16359335]

I. Information of Relaxase

ID	221
Name	BmpH_Tn5520
GenBank accession number	AAD24590
Family	MOB_V
Length	455 aa
UniProt ID	Q9X2P3
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 2.60E-22, Aligned region: 1..212
Note	relaxase
Protein sequence [Download]	MGFVVLHMEKAHGSDSGTTAHIERFIIPKNADPTHTYLNRRLIDYPDGVKDRSAAIQQRL EEAGLTRKIGSNQVRAIRINVSGTHEDMKRIEEEGRLDEWCADNLKYFADTFGKENIVAA HLHRDEETPHIHVTLVPIVKGERKRRKREEQTKKRYRKKPTNTVRLCADDIMTRLKLKSY QDTYAEAMAKYGLQRGIDGSKARHKSTQQYYRDIQKLADNLKAEVVDLQQQKETAREELR RAKKEIQTEKLKGVATVAAANIAESVGSLFGSNKVKTLERENTALHREVADHEETIEALQ DKIQTMQADHSRQMAEVERKHRREIADKETKHKEEISFLKTVIAKAAAWFPYFREMLRIE NLCRLIGFDERQTATLVKGKPLEYAGELYSEEHGRKFTTEKAGFQVVKDPTDGTRLVLAI DRKPIAEWFKEQFEKVRQNIRRPIQLQKKGRGMKL

Reference

[1] Vedantam G et al (2006) Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation. Mol Microbiol. 59(1):288-300. [PMID:16359335]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	309	_

N/A

Information of mobile element

ID	12
Element type	IME (Mobilizable transposon)
ICE description	Tn5520 (chromosomal Tn)
ICEberg accesion number	_
GenBank accession number	AF038866.2
Family	_
Genome size	4.6 kb
Coordinate of oriT [+/-]	2321..2391 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bacteroides fragilis LV23 [817]

Reference

[1] Vedantam G et al (2006) Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation. Mol Microbiol. 59(1):288-300. [PMID:16359335]