oriTDB
The information of the oriT region
oriTDB accession number 200007
Name oriT_Tn5520 (IME)  experimental
Sequence Completeness intact
oriT length 71 nt
oriT sequence CTTATTGGGGAATTTTCAGCGATACGGAGTATTGCGGCTCGGAAAATTCCCTAATAAGCT ACGGTATTTTC
IR (inverted repeat)[14-30] [55-71] nt  (CTTATTAGGGAATTTTC..GAAAATTCCCCAATAAG)
Location of nic site _
Conserved sequence flanking the
  nic site 		_
Note 71 bp minimal oriT
L:14-30;R:55-71
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Vedantam G et al (2006) Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation. Mol Microbiol. 59(1):288-300. [PMID:16359335]
I. Information of Relaxase
ID 221
Name BmpH_Tn5520 insolico
GenBank accession number AAD24590
Family MOBV
Length 455 aa
UniProt ID Q9X2P3
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 2.60E-22, Aligned region: 1..212
Note relaxase
Protein sequence [Download] MGFVVLHMEKAHGSDSGTTAHIERFIIPKNADPTHTYLNRRLIDYPDGVKDRSAAIQQRL
EEAGLTRKIGSNQVRAIRINVSGTHEDMKRIEEEGRLDEWCADNLKYFADTFGKENIVAA
HLHRDEETPHIHVTLVPIVKGERKRRKREEQTKKRYRKKPTNTVRLCADDIMTRLKLKSY
QDTYAEAMAKYGLQRGIDGSKARHKSTQQYYRDIQKLADNLKAEVVDLQQQKETAREELR
RAKKEIQTEKLKGVATVAAANIAESVGSLFGSNKVKTLERENTALHREVADHEETIEALQ
DKIQTMQADHSRQMAEVERKHRREIADKETKHKEEISFLKTVIAKAAAWFPYFREMLRIE
NLCRLIGFDERQTATLVKGKPLEYAGELYSEEHGRKFTTEKAGFQVVKDPTDGTRLVLAI
DRKPIAEWFKEQFEKVRQNIRRPIQLQKKGRGMKL
Reference
[1] Vedantam G et al (2006) Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation. Mol Microbiol. 59(1):288-300. [PMID:16359335]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 309 _ insolico
 N/A
Information of mobile element
ID 12
Element type IME (Mobilizable transposon)
ICE description Tn5520 (chromosomal Tn)
ICEberg accesion number _
GenBank accession number AF038866.2
Family _
Genome size 4.6 kb
Coordinate of oriT  [+/-] 2321..2391 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Bacteroides fragilis LV23 [817]
Reference
[1] Vedantam G et al (2006) Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation. Mol Microbiol. 59(1):288-300. [PMID:16359335]

Microbial Bioinformatics Group in MML, SJTU