oriTDB

The information of the oriT region

oriTDB accession number	200002
Name	oriT_Tn1549
Sequence Completeness	core
oriT length	28 nt
oriT sequence	GGGAGTGGCTACACTCCCTATGCTTGCT
IR (inverted repeat)	[1-7] [12-18] nt (GGGAGTG..CACTCCC)
Location of nic site	[26-27] nt
Conserved sequence flanking the nic site	RYGCTTG\|C
Note	The RYGCTTG\|C cleavage motif conserved in rolling-circle replicating (RCR) plasmids such as pNZ4000 of Lactococcus lactis and pC221 and pC223 of Staphylococcus aureus. The minimal oriT contained an inverted repeat and a core,which was homologous to the cleavage sequence found in certain Gram-positive rolling-circle replicating(RCR) plasmids.
	L:1-7;R:12-18;D:26-27

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGAGTGGCTACACTCCCTATGCTTGCT

Reference

[1] Tsvetkova K et al (2010) Analysis of the mobilization functions of the vancomycin resistance transposon Tn1549, a member of a new family of conjugative elements. J Bacteriol. 192(3):702-13. [PMID:19966009]

I. Information of Relaxase

ID	216
Name	Orf28_Tn1549
GenBank accession number	AAF72355
Family	MOB_P
Length	442 aa
UniProt ID	Q9L8Y8
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 8.20E-46, Aligned region: 9..242
Note	Rlx-like protein
Protein sequence [Download]	MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADIEFEMLLAQAYQKGNNL GHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHIDKGHVHNHIIFCAVDMVN QRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSYAEWDAQRKGTSWKAKLKAAI DAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQERFTRCKTLGEAYTEEAIKERIK GRVITRAPKERKGISLRIDLENNIKAQQSAGYERWAKLHNLKQAAKTLNFLTEHGIDTYP DLESKVAEITAASDEAAATLKAMEHRLADMAVLIKNISTYKQLWPVAMEYRNAADKAKFR REHESTLILYEAAAKALKGQGVKKLPDLYALKAEYKRLAEEKERLYEKYGEAKKQMQEYG IIKQNVDGILRMTPGKEWTQEL

Reference

[1] Grohmann E et al (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	304	Orf29_Tn1549	AAF72356	109 aa	Orf29 was a mobilization accessory component similar to MobC proteins

N/A

Information of mobile element

ID	7
Element type	ICE (Conjugative transposon)
ICE description	Tn1549
ICEberg accesion number	92
GenBank accession number	AF192329
Family	Tn916
Genome size	33805 bp
Coordinate of oriT [+/-]	22239..22266 [+]
Drug resistance	vancomycin resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterococcus faecalis BM4382 [1351]

Reference

[1] Tsvetkova K et al (2010) Analysis of the mobilization functions of the vancomycin resistance transposon Tn1549, a member of a new family of conjugative elements. J Bacteriol. 192(3):702-13. [PMID:19966009]