oriTDB
The information of the oriT region
oriTDB accession number 200002
Name oriT_Tn1549  experimental
Sequence Completeness core
oriT length 28 nt
oriT sequence GGGAGTGGCTACACTCCCTATGCTTGCT
IR (inverted repeat)[1-7] [12-18] nt  (GGGAGTG..CACTCCC)
Location of nic site [26-27] nt
Conserved sequence flanking the
  nic site
RYGCTTG|C
Note The RYGCTTG|C cleavage motif conserved in rolling-circle replicating (RCR) plasmids such as pNZ4000 of Lactococcus lactis and pC221 and pC223 of Staphylococcus aureus. The minimal oriT contained an inverted repeat and a core,which was homologous to the cleavage sequence found in certain Gram-positive rolling-circle replicating(RCR) plasmids.
L:1-7;R:12-18;D:26-27
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Tsvetkova K et al (2010) Analysis of the mobilization functions of the vancomycin resistance transposon Tn1549, a member of a new family of conjugative elements. J Bacteriol. 192(3):702-13. [PMID:19966009]
I. Information of Relaxase
ID 216
Name Orf28_Tn1549 experimental
GenBank accession number AAF72355
Family MOBP
Length 442 aa
UniProt ID Q9L8Y8
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 8.20E-46, Aligned region: 9..242
Note Rlx-like protein
Protein sequence [Download] MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADIEFEMLLAQAYQKGNNL
GHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHIDKGHVHNHIIFCAVDMVN
QRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSYAEWDAQRKGTSWKAKLKAAI
DAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQERFTRCKTLGEAYTEEAIKERIK
GRVITRAPKERKGISLRIDLENNIKAQQSAGYERWAKLHNLKQAAKTLNFLTEHGIDTYP
DLESKVAEITAASDEAAATLKAMEHRLADMAVLIKNISTYKQLWPVAMEYRNAADKAKFR
REHESTLILYEAAAKALKGQGVKKLPDLYALKAEYKRLAEEKERLYEKYGEAKKQMQEYG
IIKQNVDGILRMTPGKEWTQEL
Reference
[1] Grohmann E et al (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 304 Orf29_Tn1549 experimental AAF72356 109 aa Orf29 was a mobilization accessory component similar to MobC proteins
 N/A
Information of mobile element
ID 7
Element type ICE (Conjugative transposon)
ICE description Tn1549
ICEberg accesion number 92
GenBank accession number AF192329
Family Tn916
Genome size 33805 bp
Coordinate of oriT  [+/-] 22239..22266 [+]
Drug resistance experimental vancomycin resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Enterococcus faecalis BM4382 [1351]
Reference
[1] Tsvetkova K et al (2010) Analysis of the mobilization functions of the vancomycin resistance transposon Tn1549, a member of a new family of conjugative elements. J Bacteriol. 192(3):702-13. [PMID:19966009]