oriTDB
The information of the oriT region
oriTDB accession number 100955
Name oriT_p1002-MCR1  insolico
Sequence Completeness core
oriT length 53 nt
oriT sequence CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
IR (inverted repeat)[5-21] [25-42] nt  (CGATTGTAACATGACCG..CGGTCTTGTGTACAATCG)
Location of nic site [50-51] nt
Conserved sequence flanking the
  nic site 		TATCGTG|C
Note predicted by the oriTfinder
L:5-21;R:25-42;D:50-51
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 1025
Name Putative relaxase of p1002-MCR1 insolico
GenBank accession number WP_034169409
Family MOBH
Length 990 aa
UniProt ID A0A0R6LVD4
PDB ID _
Pfam
Note putative relaxase
Protein sequence [Download] MIVRYGGGNDGIVDYLINGRKAERQYTRDELDHRVVLDGDLQTTDKIIDSIENKSQERYL
HITLSFHESHVSNEVLKAVVDDYKKLLMNAYHPDEYSFYAEAHLPKIRHIQDNSTGELVE
RKPHIHIVIPKVNLITEKFLNPVGDVTKGHTIEQLDAIQEFINNKYNLDSPKDYPRKDAD
YGKIISRVKGDLYKEHHSELKGELLSRIENEKIENYSVFKDIVAEYGELRIRNAGKTNEY
LAVKLPGDKKYINLKSPLFRQNYIETRTLTLEKPTHKEIEKRLNTWLNKTSQEIKHIFNQ
AEKTRELYKTLSPSQQIDFLQERIKEYDSREKLNERNSQQTSGRAGGYKSCPKKFARIRQ
SEATVGLSRMPQRGMVYGINGFTRPDSVSVLSDISQRDLAEQLSQREHPGQDVRRDYDRQ
FTESGIKSLERSSFLCETMFQTLNEAAEKNEIATMAEIRRNIDPVRFLSSAAERFNIIPA
QHKIRTAKDGSPRFSVGNRNMNASDFLTKHINLAWKDAKSFLLEVYSQQLENTPYTRYPT
YRRLTHHEARERLNSLNLSEKTLRNTIRFERGKLYNDLREMRRELKLIPREQRDIAVGVI
VYKKLTTLERLSELDTEGRHIIRQYHADWHKDKDEMKALERLKSYLNFDEINAISADEPE
LSLQKAVDSQRRLEEAKKVNSKLKDLVMDKQDSRIVYRDQESEKPVFTDKGNFVVAGKNP
SKEEIGIMLEYSREKFGGVLKLTGSEDFKKMCAEVAAEQDMKIILRPEQYQQMMLELKAE
LQGNKFEQVETQENSQESESRIEKGDALKEQATEQEQATEQAQATEQAQATEQAQATEQA
QATEQEQATEQEQVTEQEQATEQEQATEQAQATEQAQATEQAQVAAQATSSYDPGVITRA
NTLDSQMLSKGTNGEFGYLKSLDSDENEIWEVLGHVPGDSDDIFDVASFDNENDAKEFCK
IVNELGIDRTQALIQEQLTTQHDQATAQVHNKQEIYCINFSRFHDLNEGIVFHSKDAAIQ
CYEESKSSAIEKYESNYLNGDGFDTVVLMSKTVSTDELSSYPEGALVTFDRPFEIIANSY
EEYRTPVYAVSFSKDEFSEDIKTFESLHDASEYKNKMLQEHGLNQDDILITPVTREEIAF
KGIKDAVNDANMAVMEQAGDSSRESPEEILASISANEHMISGLENFLVKDRSQFSSCNGD
IVVEAEITRGEGGLYHLAVAGKHGLERGDAVARVDVTEQQFAAITGKTPSEVLTGDQTSA
RVPVITGIHFSTRAIENVNKLEQQKDYVYFSTHEGLNAEIKDFSSLKDAIEWGRVECELH
DLNKRDTVIYRVESEHISQGIDAVMKNAERVERHEIEKAQGRDCTPEDGKILEAIDRFED
KFRGEGLKFEREKAESDLLNHGFTREMAEDALGKQFVQAREEHLELQQQRDNSQDMH
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1413
Plasmid name p1002-MCR1
GenBank accession number NZ_CP021205.1
Incompatibility group IncI2
Genome size 63392 bp
Coordinate of oriT  [Strand] 28384..28436 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli Z1002 [562]
Reference
[1] Zheng B et al (2016) Coexistence of MCR-1 and NDM-1 in Clinical Escherichia coli Isolates. Clin Infect Dis. 63(10):1393-1395. [PMID:27506685]

Microbial Bioinformatics Group in MML, SJTU