oriTDB
The information of the oriT region
oriTDB accession number 100911
Name oriT_pEC542_KPC  insolico
Sequence Completeness intact
oriT length 309 nt
oriT sequence CGTGTCTCCTTCTCGCCGGGGCGGGGGGCGGGGTGTGTCTTGGACAGTAAGAAGCGAGAG CGCCGCACTGTCCGGGGTCAAGGGGATACCCCTTGCAGGATAAGCCAGACCGTGAGAAGC GAAGCGCCGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAAC GGTCATCCTGTATTGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGACG CACTTTGCAACAAGCACAAATGGCGCTATGCTGTCAACTCCGCAACAAGTAAGCAAAGGA GTTAGCACG
IR (inverted repeat)IR1:[79-85] [89-95] nt  (CAAGGGGC..GCCCCTTG)
IR2: [153-160] [164-171] nt  (CACGTGGC..GCCATGTG)
Location of nic site [190-191] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note predicted by the oriTfinder
L:79-85;R:89-95;L:153-160;R:164-171;D:190-191
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 982
Name Mob_pEC542_KPC insolico
GenBank accession number WP_033996285
Family MOBP
Length 890 aa
UniProt ID A0A1J8JDJ9
PDB ID _
Pfam RepB_primase [PF16793.4], Evalue: 4.00E-70, Aligned region: 548..807
Relaxase [PF03432.13], Evalue: 3.40E-30, Aligned region: 16..274
Note putative relaxase
Protein sequence [Download] MIVKKVKNPQKAASKAVRVSRLTDYIREPERENSQEKCIHAGARGFITDEPQSQTAEMIA
LSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLH
LHLAINRVHPETLKVVKINNGFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREH
IDKDKPRQPAQPKRDMENRTGEKSAERIAIEDGAPIIKKAQTWEQLHRELAAKGMRYEKT
GSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQRQQVAQREPEPIKPDVPGWKD
YITGRKAHYAEKNAAKLALDKRQEQERKQIAEQQKARRDELMRGNWKGKGEVLNAMRSVI
AAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEG
ASGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAA
LQLSAQKWGSFTVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKS
EQLKQFELYAEAVGAERYRVTSIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLR
LQRRGENLYYTPLSDKKHHILIDDMNREKLERLIRDGYRPAVVLESSPGNYQAIITVPKL
GTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRKPKHQREDGSYPEVRLLKAE
RRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAYQRHYRDVIK
RQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARY
AYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1370
Plasmid name pEC542_KPC
GenBank accession number NZ_CP018968.1
Incompatibility group IncP(6)
Genome size 44320 bp
Coordinate of oriT  [Strand] 30126..30434 [-]
Drug resistance _
Heavy-metal resistance insolico mercury resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli Ecol_542 [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU