oriTDB
The information of the oriT region
oriTDB accession number 100904
Name oriT_pEC224_5  insolico
Sequence Completeness intact
oriT length 83 nt
oriT sequence GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA ACCATGCGGCATCAGTGCGGATT
IR (inverted repeat)[3-12] [16-25] nt  (GTGTCGGGGC..GCCCTGACCC)
Location of nic site [56-57] nt
Conserved sequence flanking the
  nic site 		CTGG|CTTA
Note predicted by the oriTfinder
L:3-12;R:16-25;D:56-57
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 975
Name Putative relaxase of pEC224_5 insolico
GenBank accession number WP_024623332
Family MOBP
Length 517 aa
UniProt ID A0A1U9SBD2
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 7.50E-20, Aligned region: 49..246
Note putative relaxase
Protein sequence [Download] MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF
AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL
QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDA
LYHAGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFADGRGV
REKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRRYSPVHSLDRGIAGKTPGRGE
RGDDAAQEGRVKAGREYGHDVTGDSISPVYREWRDALVSWREDTGEPGRNQEAGRDIAET
EREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREL
EIREERLIQEREHVLSLERERQPEIQERTLDGPSLGW
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1363
Plasmid name pEC224_5
GenBank accession number NZ_CP018943.1
Incompatibility group ColRNAI
Genome size 6647 bp
Coordinate of oriT  [Strand] 5449..5531 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli Ecol_224 [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU