oriTDB
The information of the oriT region
oriTDB accession number 100852
Name oriT_pKPGJ-3d  insolico
Sequence Completeness core
oriT length 106 nt
oriT sequence AGTATGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCATCCAAAAACGT CTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
IR (inverted repeat)[18-35] [41-58] nt  (GTTTTTGGAGTACCGCCG..CGGCGGCCATCCAAAAAC)
Location of nic site _
Conserved sequence flanking the
  nic site
_
Note predicted by the oriTfinder
L:18-35;R:41-58
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 924
Name Putative relaxase of pKPGJ-3d insolico
GenBank accession number WP_077138896
Family MOBP
Length 659 aa
UniProt ID A0A1Q1E692
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 4.50E-31, Aligned region: 13..334
Note putative relaxase
Protein sequence [Download] MVIPKIIEGRRDKKSSFAQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLIR
KQKVISTPVDVEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQNTRCKDPVMHYVLSW
PDYEKPNDDQVFDSVKFTLASMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSS
FSKDTLHQACRLLELKNGWSHSNGAYVVNERQQIVRNPHSKKERGNWRSLDRINKMENKE
GVETLYRYIVGDEQVGGSRQNFIHVSAGLREAKSWDDVHKTFADIGLRFEKAQGKKGYVI
THEHQNLKTAVKASLVFNKAQYTLKSMEERFGEYQPSRIEPAKVSVFKTAYTPGAYRRDA
NKRLQRKIERAEERMLLKGRYRAYRNNLPTYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPYTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAAL
SQMRGFAYSSRKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGT
VIFERDGKPVAADRGHIVLTESNGIDKEKTADLAIALTIAGKAKSLRVDGDGEFKELCCN
RIVDAAVNHNHPVAQGITFTDAAQQAYAQNEKHRLIREQNNSKNEMQFRSENDDKFKPK
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1312
Plasmid name pKPGJ-3d
GenBank accession number NZ_CP017288.1
Incompatibility group IncL/M(pOXA-48)
Genome size 58665 bp
Coordinate of oriT  [Strand] 14402..14507 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Klebsiella variicola GJ3 [244366]
Reference
[1] Di DY et al (2017) Emergence of Klebsiella variicola positive for NDM-9, a variant of New Delhi metallo-β-lactamase, in an urban river in South Korea. J Antimicrob Chemother. 72(4):1063-1067. [PMID:28087584]