oriTDB
The information of the oriT region
oriTDB accession number 100731
Name oriT_pDM01  insolico
Sequence Completeness intact
oriT length 110 nt
oriT sequence TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATA GCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
IR (inverted repeat)IR1: [24-31] [35-42] nt  (GTCGGGGC..GCCCTGAC)
IR2: [49-65] [72-88] nt  (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC)
Location of nic site [96-97] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note predicted by the oriTfinder
L:24-31;R:35-42;L:49-65;R:72-88;D:96-97
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 803
Name NikB_pDM01 insolico
GenBank accession number WP_069924919
Family MOBP
Length 899 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 9.20E-64, Aligned region: 47..315
Note putative relaxase
Protein sequence [Download] MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRL
RNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHY
ILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYL
NRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTF
REYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGKFLVMDGWDRNREGVQLDSFG
PSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHL
GERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKREL
FSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHE
QTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDP
VLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDP
ALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRASVS
QLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGE
FVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1191
Plasmid name pDM01
GenBank accession number NZ_CP012039.1
Incompatibility group IncI1
Genome size 109715 bp
Coordinate of oriT  [Strand] 70231..70340 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Salmonella enterica subsp. enterica serovar Ouakam strain GNT-01 [1243585]
Reference
[1] Marasini D et al (2016) Whole-Genome Sequencing of Salmonella enterica subsp enterica Serovar Ouakam Isolated from Ground Turkey. Genome Announc. 4(1). [PMID:26798110]

Microbial Bioinformatics Group in MML, SJTU