oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100701
Name	oriT_pAcX50c
Sequence Completeness	core
oriT length	90 nt
oriT sequence	GTGAAGATAGATAACCGGCTTGCCGGTTAGCTAACTTCACCTGTCTTGCCCGGCTCTTCG AGCCGTTTAACGCCATATAAGTATCGCATA
IR (inverted repeat)	[1-19] [22-40] nt (GTGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCAC)
Location of nic site	[48-49] nt
Conserved sequence flanking the nic site	TGTCTTG\|C
Note	predicted by the oriTfinder
	L:1-19;R:22-40;D:48-49

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	773
Name	TraI_pAcX50c
GenBank accession number	WP_040107071
Family	MOB_P
Length	799 aa
UniProt ID	A0A0C4WL02
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.70E-50, Aligned region: 12..252
Note	putative relaxase
Protein sequence [Download]	MISKRIPMNSAKKSSFAGLVNYITNSQGISERVGEVRITNCQSESVTWAVRDILSAQQKN TRAEGDKTYHLLISFAPGEKPSAEVLRDVEDRICAAMGYGEHQRISAVHNDTDVLHIHVA INKIHPQRLTMHEPYRDFKTRAEICAKLEIEHGLERVNHTGRKSLSESKANDMERHAGVE SLLSWVRRECLEQIQNATSWKEMHQVLHEHGLELRKRGNGLVIADHEGTMVKASTVARDL SKNKLEDRLGEFEGRQHGRPTPSPGTPLPKPGIGKVGRRPPPASRNRLRSMSSLQSLKID SGKRYEKRPVGHRTDTTELYARYKDEQSAVRHTRAKELAAERDRRDRALENARRAADLKR ASIKLMVAPGLNKKLLYASTHKTLKEQIAKIQTEHRKAVEKINTTRKARQWADWLQVKAK EGDQEALKALRARAGVKGLQGDAIAAKGPQKPSQAVLAGQDHITKEGTVIYLAGAAAIRD DGSKLQVSRGTTFDGIETALRMAVARYGKQVTVTGSPQFKELVAQTAAVRNLPIKFDDPA LEQRRQALQQAIEKERNNVRADRGRAAGTSAGGNGFDPARRGRSDTGRTTGAADGQGDGV RGSATGTGVPERLGAVDGGRIPVRALGGTAKPGVARVGRKPPPESQNRLRALSQLGVVQL THGAEVLLPRHVPGDVEHQGAKPTDGLRRDVSRPGVAAPTSPISGQQAARIYIEEREEKR AKGFDIPKHRLYNQADDGAASFEGVRQVKGQFMALLKRGDDVLVLPINEATARRMKRMKL GDPVRATAKGAIKSKGKSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1161
Plasmid name	pAcX50c
GenBank accession number	NZ_CP010418.1
Genome size	62783 bp
Coordinate of oriT [Strand]	12642..12731 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Azotobacter chroococcum NCIMB 8003 [1328314]

Reference

[1] Robson RL et al (2015) Azotobacter Genomes: The Genome of Azotobacter chroococcum NCIMB 8003 (ATCC 4412). PLoS One. 10(6):e0127997. [PMID:26061173]