 oriTDB accession number | 100691 |
| Name | oriT_pECC-1470_100  |
Sequence Completeness  | intact |
| oriT length | 462 nt |
| oriT sequence | AAGAAGAGAACCCCTAGCAGCGCCCCTAGGGGCATACTATAAAAAAACGCACGGCGCTGC TAGGGGCGGCCCTAATATATCCAAATGTTTTTCATGAAAATTGTCAGCACTGACCCTAAC AAGGGTCGTCATAGGGTCGCTATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAA ATTAAAATACATTATCTAGAACATAAACTAATGATTTAAAAAACAAATCAGCAAAAACTT GTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTT GTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGG ATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCCATGCCACTGATTGCTTTGAT CTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGAGA |
| IR (inverted repeat) | [142-151] [154-163] nt (ATAGGGTCGT..ACGACCCTAT) [231-238] [241-248] nt (GCAAAAAC..GTTTTTGC) |
| Location of nic site | [257-258] nt |
| Conserved sequence flanking the nic site | GTGGGGTGT|GG |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 763 |
| Name | TraI_pECC-1470_100 |
| GenBank accession number | WP_041521561 |
| Family | MOBF |
| Length | 1756 aa |
| UniProt ID | _ |
| PDB ID | _ |
| Pfam | TrwC [PF08751.10], Evalue: 1.30E-74, Aligned region: 10..284 TraI [PF07057.10], Evalue: 1.30E-56, Aligned region: 1434..1556 AAA_30 [PF13604.5], Evalue: 1.70E-34, Aligned region: 968..1160 AAA_19 [PF13245.5], Evalue: 7.40E-09, Aligned region: 973..1108 |
| Note | putative relaxase |
| Protein sequence [Download] | MMSIAQVRSAGSAGNYYTDKDNYYVLGCMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRL PDGADLSRMQDGRNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIEAHNQAVDFAVRQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKV GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQT IREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRDVADQRAE TRTQAPGPASPDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERALAG IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE RLSGELITGRRQLQEGMAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ VSGVREQAILTQAIRSELKTQGVLGRPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN PETRSHDRYVIDRVTAQSNSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFMALKLENGWVETPGH SVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI KARAGETLLETAISLQKAGLHTPAQQAIHLALSVVESKNLAFSMVDLLTEAKSFAAEGTS FVDLGREINTQIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMER VPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPERERPRV VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV YSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKSMLKGEA FPDVPMTLYEAIVRDYTGRTPKAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQEQVM VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT RVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMK QHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDVAAGRAV LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGTIT GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP DGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREVARENLLQERLQQME RDMVRDLQKEKTLGGD |
Reference| ID | 1151 |
| Plasmid name | pECC-1470_100 |
| GenBank accession number | NZ_CP010345.1 |
| Incompatibility group | IncFIB(AP001918) |
| Genome size | 100061 bp |
| Coordinate of oriT [Strand] | 64638..65099 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli ECC-1470 [758831] |
Reference| [1] Leimbach A et al (2015) Complete Genome Sequences of Escherichia coli Strains 1303 and ECC-1470 Isolated from Bovine Mastitis. Genome Announc. 3(2). [PMID:25814601] |