oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100558
Name	oriT_pACN001-C
Sequence Completeness	intact
oriT length	83 nt
oriT sequence	GGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA ACTATGCGGCATCAGTGCAGATT
IR (inverted repeat)	[3-12] [16-25] nt (GTGTCGGGGC..GCCATGACCC)
Location of nic site	[56-57] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:3-12;R:16-25;D:56-57

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	630
Name	MbeA_pACN001-C
GenBank accession number	YP_008998316
Family	MOB_P
Length	517 aa
UniProt ID	A0A140WYV4
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.60E-20, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAESVTRGIDA LYHAGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGRNIRLKGAFYEQSFTDGRGV REKAERESRIYRENAERRVQEARRVCQRGCDLKRDENQRRYSPVHSLDREIAGKTPGRGE RGDDAAQEGRVKAGREYGHDVTGDSLSPVYHEWRDALVSWRTDTDEPGRNQEAGRDIAET EREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREL EIREERLIQEREHVLSLERERQPEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1018
Plasmid name	pACN001-C
GenBank accession number	NC_023324.1
Incompatibility group	ColRNAI
Genome size	5784 bp
Coordinate of oriT [Strand]	5494..5576 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli ACN001 [1311757]

Reference

[1] Wang X et al (2014) Plasmid-mediated multidrug resistance and virulence in an avian pathogenic Escherichia coli strain isolated in China. J Glob Antimicrob Resist. 2(1):57-58. [PMID:27873640]