oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100523
Name	oriT_pJEG011
Sequence Completeness	core
oriT length	106 nt
oriT sequence	AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGT CTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
IR (inverted repeat)	[18-35] [41-58] nt (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:18-35;R:41-58

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	594
Name	MobA_pJEG011
GenBank accession number	YP_007878553
Family	MOB_P
Length	658 aa
UniProt ID	G9I9E5
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 5.10E-31, Aligned region: 12..333
Note	putative relaxase
Protein sequence [Download]	MIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRK QKVISTPVDVEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWP DYEKPNDDQVFDSVKFTLASMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSF TKDTLHQACRLLELKNGWSHSNGAYVVNDRQQIVRNPHSKKERGNWRSLDRINKMENKEG VETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHKTFADIGLRVEKAQGKKGYVIT HEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTAYTPGAYRRDAN KRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSVS DPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALS QMRGFAYSSRKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTV IFERDGKPVAADRGHIVLTESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNR IVDAAVNHNHPVAQGITFTDAAQQAYAQNEKHRLIREQNNSKNEMQFRSESDDKFNPK

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	984
Plasmid name	pJEG011
GenBank accession number	NC_021078.1
Incompatibility group	IncL/M(pOXA-48)
Genome size	71446 bp
Coordinate of oriT [Strand]	42510..42615 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Klebsiella pneumoniae Kp002 [573]

Reference

[1] Espedido BA et al (2013) Whole genome sequence analysis of the first Australian OXA-48-producing outbreak-associated Klebsiella pneumoniae isolates: the resistome and in vivo evolution. PLoS One. 8(3):e59920. [PMID:23555831]