oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100501
Name	oriT_pEl1573
Sequence Completeness	core
oriT length	106 nt
oriT sequence	AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGT CTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
IR (inverted repeat)	[18-35] [41-58] nt (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:18-35;R:41-58

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	572
Name	MobA/NikB_pEl1573
GenBank accession number	YP_006965403
Family	MOB_P
Length	658 aa
UniProt ID	J7KBG3
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 5.10E-31, Aligned region: 12..333
Note	putative relaxase
Protein sequence [Download]	MIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRK QKVISTPVDVEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWP DYEKPNDDQVFDSVKFTLASMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSF TKDTLHQACRLLELKNGWSHSNGAYVVNDRQQIVRNPHSKKERGNWRSLDRINKMENKEG VETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHKTFADIGLRVEKAQGKKGYVIT HEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTAYTPGAYRRDAN KRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSVS DPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALS QMRGFAYSSRKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTV IFERDGKPVAADRGHIVLTESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNR IVDAAVNHNHPVAQGITFTDAAQQAYAQNEKHRLIREQNNSKNEMQFRSERDDKFNPK

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	962
Plasmid name	pEl1573
GenBank accession number	NC_019368.1
Incompatibility group	IncL/M
Genome size	87731 bp
Coordinate of oriT [Strand]	28558..28663 [+]
Drug resistance	resistance to gentamicin and tobramycin; resistance to chloramphenicol; resistance to sulphonamides
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterobacter cloacae El1573 [550]

Reference

[1] Partridge SR et al (2012) pEl1573 Carrying blaIMP-4, from Sydney, Australia, is closely related to other IncL/M plasmids. Antimicrob Agents Chemother. 56(11):6029-32. [PMID:22926566]