oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100488
Name	oriT_pNDM-BJ02
Sequence Completeness	incomplete
oriT length	38 nt
oriT sequence	AGGGATTCATAAGGGAATTATTCCCTTATGTGGGGCTT
IR (inverted repeat)	[8-17] [21-30] nt (CATAAGGGAA..TTCCCTTATG)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:8-17;R:21-30

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	559
Name	TraA_pNDM-BJ02
GenBank accession number	YP_006961792
Family	MOB_Q
Length	996 aa
UniProt ID	H6VYP3
PDB ID	_
Pfam	AAA_30 [PF13604.5], Evalue: 3.70E-45, Aligned region: 371..567 MobA_MobL [PF03389.14], Evalue: 1.50E-26, Aligned region: 47..237 AAA_19 [PF13245.5], Evalue: 1.70E-10, Aligned region: 376..503 Viral_helicase1 [PF01443.17], Evalue: 1.60E-05, Aligned region: 617..736
Note	putative relaxase
Protein sequence [Download]	MTIAVGMHASAFLSEKQKKQKQKRHKMAIYHLSVKTGRQYGSTGTRGANHYAYINRDGKY KRDDLENIKSGNLPSWATDAKHFWKSADKNERINGRVYTELEISLPRELNKEQREELVEK FVEKTLGENFTYSYAIHSPLASDGQQNPHVHLMFSERKLDGIERDEVKHFKRYNPVNPEK GGAGKDRYFSSKMFVVDTRLEWANHVNDFCENLGIDARIDHRSFKEQGIELSSQNFRADY VSSHKYHINENIKQVRHQNGEIIIERPSEAIKALTSNQSVFSVRELERFLMAHTDGEEQY LKAYESVITCPDMATLYGKDKVVFSSKELVGIEESISTFIYNANEESRIQKPFNLVEKLP LNAVRVAETRTFNSEQEKAFYTLTSTDRITLLNGSAGTGKSYVLSAVSEAYKESDYQVYG IALQAITAKAIANDCDIPSSTIASFLSKYDSGNLEINNKTVLILDEAGMVGSRDMQRLLM LVEKHDAQIKLVGDSYQLSAVSAGHVFANIQENLDKKNIASLEDIQRQKHAKESDKMIQA SKYLSKHEVGKAIDIYKSINMVNEYESHDLALAKTVKSWSEDSSESKLMLAHSNSDVNEL NRMARALLIKQGKLNPESVSVKNYQGDLDISIGEKIVFKQNNSQMKVSNGELAKVIGFEK DKFNNPKAMMVQMESDSRIVRVDLKEYDQFKHGYANTIHSSQGMTVDSAYIVASENMNAN LTYVALTRHKQNIQINYSALTFATNEKETVKTPDGKVSYDHNYKPVEREVTAFENFKKVL GRSETKEFSTDFTLVESKDNLMKSYLNDHRLHLENKRELFGINSKLYSLTSENKTFTKEE IVSEVRASLKSKALLGDEIVKFNGNMNIAKGELVKLTNDLEIKTGLFKKVTFEKGTELKV IDAYKVKDKPVMKARIKNEEYEIPFNKLNLEYSDKYFNEFKDQSKARFDSRHKTEVYTEF REQLLKQKANAPKDNLNAPNQRIKNPNTPNRNTPKY

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	949
Plasmid name	pNDM-BJ02
GenBank accession number	NC_019281.1
Genome size	46165 bp
Coordinate of oriT [Strand]	259..296 [+]
Drug resistance	aminoglycoside resistance; bleomycin resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Acinetobacter lwoffii WJ10659 [28090]

Reference

[1] Hu H et al (2012) Novel plasmid and its variant harboring both a bla(NDM-1) gene and type IV secretion system in clinical isolates of Acinetobacter lwoffii. Antimicrob Agents Chemother. 56(4):1698-702. [PMID:22290961]