oriTDB
The information of the oriT region
oriTDB accession number 100455
Name oriT_p417H-90  insolico
Sequence Completeness intact
oriT length 450 nt
oriT sequence ATATGTATACCTCTTTATTTTCTTATCGCATCAAATTTATTTCATATATAAAAAAATGAC AATTCATCGATGAATCGAATCGTGACGCAAGTTACGAAAATTACGATTCATGCAGCAAAT CACATCACGAATTGAATCTAGAGTCAATTCGCTTTAACTCGTTGTTTTATTATTACTGAT TCATCTATGAGTTGTGTTAAATTCATTGGTGAATCATATGCGATTCACCAATGAATCCTT TTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAG CAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCA ACTAGTTGAGCCTTATTGTGGAGTAGGTTAAATTATTTCCGGATAATGTCACCAGAGGTG AAAAAATGAAAAAGTGGATGTTAGCAATCT
IR (inverted repeat)IR1: [201-215] [222-236] nt  (ATTCATTGGTGAATC..GATTCACCAATGAAT)
IR2: [308-315] [318-325] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [334-335] nt
Conserved sequence flanking the
  nic site 		GTAGTGTGT|GG
Note predicted by the oriTfinder
L:201-215;R:222-236;L:308-315;R:318-325;D:334-335
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 526
Name TraI_p417H-90 insolico
GenBank accession number YP_006954170
Family Other
Length 1640 aa
UniProt ID I3W000
PDB ID _
Pfam TraI [PF07057.10], Evalue: 2.00E-56, Aligned region: 1318..1440
TrwC [PF08751.10], Evalue: 4.40E-39, Aligned region: 1..168
AAA_30 [PF13604.5], Evalue: 1.50E-34, Aligned region: 852..1044
AAA_19 [PF13245.5], Evalue: 6.80E-09, Aligned region: 857..992
Note putative relaxase
Protein sequence [Download] MEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHAVVANVTQHNGEWKTLS
SDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVVGKHGMWEMPGVPVEAFSG
RSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMAEWMQTLKETGFDIRAYRDAAD
QRADLRTLTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPEAGVIER
ARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRT
AGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNL
KQDERLSGELITGRRQLQEGMTFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITD
SGQRTGTGSALMAMKDAGVNIYRWQGGEQRPTTIISEPDRNVRYARLAGDFAASVKAGEE
SVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVM
EQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGE
RLRVTGKIPGLRVSGGDRLQVTSVSEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVE
TPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVV
SEQIKTRAGETSLETAISHQKSALHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAA
EGTSFTDLGGEINAQIKRGDLLHVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTP
LMERVPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPERE
RPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVG
NTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPEL
REAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMQ
KGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGK
EQVMVPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNT
RLISPREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSD
GQKTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDVAA
GRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGNGLRG
FSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNP
GAITGGRVWGDIPDNSVQPGAGNGEPLTAEILAQRQAEEAIRRETERRADEIVRKMAENK
PDLPDGKTEQAVREIAGQERDQAVTSEREAALPESVLREPQREREAVREIARENLLQERL
QQMERDMVRDLQKEKTLGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 916
Plasmid name p417H-90
GenBank accession number NC_019094.1
Incompatibility group IncFIC(FII)
Genome size 79478 bp
Coordinate of oriT  [Strand] 77317..77766 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli 417H [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU