oriTDB
The information of the oriT region
oriTDB accession number 100402
Name oriT_pCV56C  insolico
Sequence Completeness intact
oriT length 463 nt
oriT sequence TTAGTGGATGAACAAAAAAATTCGAAAGATTTTTTGTTCTTTCATCCATGATTTTAGAAA AAAGAGGGACGATTTCAGAAGACGAAATTCGTCTCTTTTTTTCTTCTTTTTGTATGACAA AAAGAAATATCTTTTGCCCATTTTATTTTTATCAAATGGGCAGCTGGCGTTTGCGTAAAG CAAATCAACACAATCCAAAGGGGATAAAAGTGGAAAGTGAAACTTCCCCCTTTTCAAGCC ACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTAATAGCTTACCAGTATTTAT GGTTTTATATTTTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTA CAAAATCAATGACAAAGGGCGATTGACTAATGAGCGCTGAGGCATTTTATCTTTGAGGAA GTTCTTGATGGATCAGAAAAATGTATCACAAATTTAAACAAAG
IR (inverted repeat)IR1: [137-146] [151-160] nt  (CCCATTTTAT..ATCAAATGGG)
IR2: [206-218] [222-234] nt  (AAAAGTGGAAAGT..ACTTCCCCCTTTT)
IR3: [241-254] [267-280] nt  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site _
Conserved sequence flanking the
  nic site 		_
Note predicted by the oriTfinder
L:137-146;R:151-160;L:206-218;R:222-234;L:241-254;R:267-280
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 473
Name PriA_pCV56C insolico
GenBank accession number WP_014570287
Family MOBP
Length 505 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.90E-50, Aligned region: 16..262
Note putative relaxase
Protein sequence [Download] MTVIYMPKQSNGTVHSSKDLNQLIDYVMNPEKTNDFEYVSGQNILDIHSTCDEMLATRTM
ANALKNKPQKNEIFGYHFVQSFSPDDHLTPEQVHEIGLKTMKEYLGSSAEFIIATHTDKP
HLHNHIVLNATDPLTLNKFQQNKNDLERLKEISDKISKEYGCKIIVRPEQKLGNSHKNYL
VYLAKNSYRKEIKNKLEFLMNHSHTWEDFKTKARALNLKVDDTKKYTTYLLEGSEQTKKI
RDRSLKNDKFLKENLKERIEKNTIGYSVDEVVKLWKDKESIQEKNQEKEISILVEDWQIE
KETENFLYVTIDTALDKEATIKIPARCVDKLENGDYQVFIKNKDRFSYLDKKSPANNKIM
YGTTVAKNLQRQSGNVPLYSDNANIKLKQVFHEFDFLISQGLGFDRSFETIGKDLKATYQ
ETEKQLDKLDTKIIEYVEQNKSLDYVDTTIRDTIKNLKLERDDLRDTLYKVDKNIQYYQK
SEQRLEAYQKNQSPKNKARDDDFEI
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 863
Plasmid name pCV56C
GenBank accession number NC_017484.1
Genome size 31442 bp
Coordinate of oriT  [Strand] 23520..23982 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis subsp. lactis CV56 [929102]
Reference
[1] Gao Y et al (2011) Complete genome sequence of Lactococcus lactis subsp lactis CV56, a probiotic strain isolated from the vaginas of healthy women. J Bacteriol. 193(11):2886-7. [PMID:21460077]

Microbial Bioinformatics Group in MML, SJTU