oriTDB
The information of the oriT region
oriTDB accession number 100353
Name oriT_pPR9  insolico
Sequence Completeness core
oriT length 36 nt
oriT sequence CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
IR (inverted repeat)[4-10] [14-20] nt  (GCGAACG..CGTTCGC)
Location of nic site [31-32] nt
Conserved sequence flanking the
  nic site
TAAGTGCGCC|CT
Note predicted by the oriTfinder
L:4-10;R:14-20;D:31-32
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 424
Name Nes_pPR9 insolico
GenBank accession number YP_003347965
Family MOBQ
Length 665 aa
UniProt ID D2KNY7
PDB ID _
Pfam MobA_MobL [PF03389.14], Evalue: 8.20E-55, Aligned region: 17..218
Note putative relaxase
Protein sequence [Download] MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADD
KFKDREYLWNKVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDL
NIHKINEENPHAHLLCTLRGLDKNNEFEPKRKGNSYIRDWNTKEKHNEWRKRWENVQNKH
LEKNGFSVRVSADSYKNQNIDLEPTKKEGWKARKFEDETGQKSSISKHNESIKKRNQQKI
DKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYVTPINMYEESERLYDLKQKTA
LITDDEDRLNKIENIEDRQRKLESINEVFEKQSKIFFDKNYPDQKLNYSDDEKIFITRTI
LNDRDVLPANHELEDIVKEKRIKEAQISLNTVIGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVLRNAENTLEDYYDVQIKELFTDDEDYKAF
NEVTDIKEKQQLIDFKTYHGSENTLEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPN
KNQHDKFVLGAIQKKLLSEYDFDYSDNNDLKHLYQESYEVGDEISKDNIEEFYEGNDILV
DKETYNNYNKKQQAYGLVNAGLDSMIFNFNEIFRERMPKYINHQYKGKNHSKQRHELKNK
RGMHL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 814
Plasmid name pPR9
GenBank accession number NC_013653.1
Genome size 41715 bp
Coordinate of oriT  [Strand] 20522..20557 [+]
Drug resistance experimental mupirocin resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Staphylococcus aureus HUNSC491 [1280]
Reference
[1] PĂ©rez-Roth E et al (2010) Complete nucleotide sequence and comparative analysis of pPR9, a 417-kilobase conjugative staphylococcal multiresistance plasmid conferring high-level mupirocin resistance. Antimicrob Agents Chemother. 54(5):2252-7. [PMID:20211895]