 oriTDB accession number | 100342 |
| Name | oriT_pO103  |
Sequence Completeness  | intact |
| oriT length | 290 nt |
| oriT sequence | CGCACCGCTAGCAGTGCCCCTGGCGGTATCCTATAAAGAAACACACCGCGCCGCTAGCAG CACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACTACCCCTACAAAGCGG TGTCGGTGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAA GAGAAACTAATTTTTCATAAAACTCTATTTATAAATAAAAATCAGCAAAAACTTGTTTTT GCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAATCTGTTGAGCCT |
| IR (inverted repeat) | [225-232] [235-242] n (GCAAAAAC..GTTTTTGC) |
| Location of nic site | [251-252] nt |
| Conserved sequence flanking the nic site | GTGGGGTGT|GG |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 413 |
| Name | TraI_pO103 |
| GenBank accession number | WP_000987003 |
| Family | MOBF |
| Length | 1756 aa |
| UniProt ID | A0A140RHP8 |
| PDB ID | _ |
| Pfam | TrwC [PF08751.10], Evalue: 6.10E-75, Aligned region: 10..284 TraI [PF07057.10], Evalue: 4.30E-55, Aligned region: 1434..1556 AAA_30 [PF13604.5], Evalue: 2.70E-34, Aligned region: 968..1160 AAA_19 [PF13245.5], Evalue: 1.30E-08, Aligned region: 973..1108 |
| Note | putative relaxase |
| Protein sequence [Download] | MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRL PDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKV GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQA IREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMTEWMQTLKETGFDIRAYRDAADQRAE TRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARAG IDEAISREQLIPLDREKGLFTSGIHVLNELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ VSGVREQAILTQAIRSELKTQGVLGHQEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN PETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV TGKISGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGH SVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTS FADLGREINTQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMER VPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNLLPESERPRV VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV YSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEA FPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM VPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT RVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGGRKQMAGFESAYVALSRMK QHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPGSDREVMNAERLFSTARELRDVVAGRAV LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP DGRTEQAVREIAGQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQERLQQME RDMVRDLQKEKTLGGD |
Reference| ID | 803 |
| Plasmid name | pO103 |
| GenBank accession number | NC_013354.1 |
| Incompatibility group | IncFIB(AP001918) |
| Genome size | 75546 bp |
| Coordinate of oriT [Strand] | 72592..72881 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli O103:H2 str. 12009 [585395] |
Reference| [1] Ogura Y et al (2009) Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli. Proc Natl Acad Sci U S A. 106(42):17939-44. [PMID:19815525] |