oriTDB
The information of the oriT region
oriTDB accession number 100335
Name oriT_pEP05  insolico
Sequence Completeness intact
oriT length 179 nt
oriT sequence TTAGGGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTG GCTTAGTATGTTGCCACGGCGTTCTGTTTCATGGAAGTGCTTCATGTGGTGGTGAAAAAG GCGGATTCGGAGTGACAGGGGAATATGCAGGCTCAGGTGATATGACGCCTCCTCGTTCA
IR (inverted repeat)[10-17] [21-28] nt  (TTCGGGGC..GCCCTGAA)
Location of nic site [61-62] nt
Conserved sequence flanking the
  nic site 		CTGG|CTTA
Note predicted by the oriTfinder
L:10-17;R:21-28;D:61-62
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 406
Name Nuclease_pEP05 insolico
GenBank accession number WP_012814659
Family MOBP
Length 504 aa
UniProt ID D2TE66
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 2.40E-15, Aligned region: 47..247
Note putative relaxase
Protein sequence [Download] MIVKFHPRGRGGGSGPVDYLLGKDRQRDGASVLQGKPEEVLELIDASAYAKKYTSGVLSF
AEKDLAPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLIPNTELLTGRRL
QPYYDRADRPRIDAWQTVVNGRLGLHDPNAPENRRALVTPSALPKAKLEAAETITRGLLA
IASSGELKTRQDVTEALESAGFDVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGL
RAEIESAAAEYRRDAESRIQRAREVCKSGTERKREENQRRHSRPRPAAVLSHEPAHERHG
THGQPDVADHRPGFRPVDRLERGHSVVAGAADTRQLHEHPGTEGRAGEAERADMGREISG
RQPGAFSGAAGRRESGHELDVRQRQEERHQTGTGVEQHDGAGKTVAERIRAATAGLLEKA
GRVGERLRGMADDVWSYATGERGAERARHGFEQAGAEFERAAAPVVVKLNGIEAHRQQER
AIQQQKELELERSQRQRTYDGPSL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 796
Plasmid name pEP05
GenBank accession number NC_013265.1
Genome size 4955 bp
Coordinate of oriT  [Strand] 2..180 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Erwinia pyrifoliae Ep1/96 [634499]
Reference
[1] Kube M et al (2010) Genome comparison of the epiphytic bacteria Erwinia billingiae and E tasmaniensis with the pear pathogen E pyrifoliae. BMC Genomics. 0.73125. [PMID:20565991]

Microbial Bioinformatics Group in MML, SJTU