oriTDB
The information of the oriT region
oriTDB accession number 100330
Name oriT_pSRC15  insolico
Sequence Completeness intact
oriT length 160 nt
oriT sequence GGCCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCCCTACAAAGTAGGGTCGGGAT TGCCGCCGCTGTGCCTCCATGATAGCCTACGAGACAGCACATTAACAATGGGGTGTCAAG ATGGTTAAGGGGAGCAACAAGGCGGCGGATCGGCTGGCCA
IR (inverted repeat)IR1: [3-12] [16-25] nt  (CCAGTTTCTC..GAGAAACCGG)
IR2: [39-44] [48-53] nt  (CCCTAC..GTAGGG)
Location of nic site [33-34] nt
Conserved sequence flanking the
  nic site 		TAAGTGCG|CCCT
Note predicted by the oriTfinder
L:3-12;R:16-25;L:39-44;R:48-53;D:33-34
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 401
Name MobA_pSRC15 insolico
GenBank accession number YP_003104762
Family MOBQ
Length 709 aa
UniProt ID C7FHN5
PDB ID _
Pfam RepB_primase [PF16793.4], Evalue: 1.80E-47, Aligned region: 396..643
MobA_MobL [PF03389.14], Evalue: 2.00E-33, Aligned region: 13..218
Note putative relaxase
Protein sequence [Download] MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESGHMPEFVERPADYWDA
ADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAERLPYTLAIHAGGGENPH
CHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPKAWLEQTREAWADHANRA
LERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRTDRADVALNIDTANAQIID
LQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGRRSPAGHEPDPAGQRGAGGG
VAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGVALEAMANRDAGFHDAYGGAA
DRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQM
MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREP
ALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKD
KHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLS
RHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAER
KPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 791
Plasmid name pSRC15
GenBank accession number NC_013104.1
Incompatibility group IncQ1
Genome size 8688 bp
Coordinate of oriT  [Strand] 6261..6420 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Salmonella enterica subsp. enterica serovar Typhimurium [90371]
Reference
[1] Yau S et al (2010) RSF1010-like plasmids in Australian Salmonella enterica serovar Typhimurium and origin of their sul2-strA-strB antibiotic resistance gene cluster. Microb Drug Resist. 16(4):249-52. [PMID:20617928]

Microbial Bioinformatics Group in MML, SJTU