oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100320
Name	oriT_pCHE-A
Sequence Completeness	core
oriT length	88 nt
oriT sequence	GGCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCCGTCAGAATGACGGTCGGGGTT TGCAGCGTCTGTGCTCCGATGATAGCAT
IR (inverted repeat)	[5-10] [16-21] nt (GTTTCT..AGAAAC)
Location of nic site	[32-33] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	predicted by the oriTfinder
	L:5-10;R:16-21;D:32-33

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	391
Name	MobA_pCHE-A
GenBank accession number	YP_002563156
Family	MOB_Q
Length	701 aa
UniProt ID	B9TSG1
PDB ID	_
Pfam	RepB_primase [PF16793.4], Evalue: 5.90E-48, Aligned region: 394..640 MobA_MobL [PF03389.14], Evalue: 8.70E-33, Aligned region: 16..218
Note	putative relaxase
Protein sequence [Download]	MAIYHLTAKTGSRNGGQSAKAKADYIQREGRYSRDRDEVLHTQSGHLPKWAERPADYWDV ADLYERANGRLFKEVEFALPVELTLDQQRELVDEFARHLTDGERLPYTLAIHAGDGENPH CHLMISERKNDGIERPADQWFKRYNAKQPERGGAQKSESLKPKAWLEQTREAWSHHANRA LERAGHEARIDHRTLEAQGIERLPGIHLGPNVVEMEGRGIRTERADTALSIDTANGQIID LQEYREAIEHERDRQSEEIQRDQRVSGRDRAVGPEHGDTGGRSPAADRRGTAGQRGAGRE LDEPATASHERMAGGSQGHEDSRRGADHGQPDGPERRPWLDVAAMGRSLDRFRDAYSGAA DRIMALAGAARNRATGGHMADFQAQVKSDRTAQAIARQLKAMGCDRYDIGIRDAASGKMM NREWTPQEVQQNADWLKRMNAQGNDIYIRPAEQARHGLVLVDDLSSDDLDAMKQEGREPA AIIETSPRNYQAWVKVAQDAPAVHRGVIARELAREYDADPASADSRHYGRLAGFTNRKDK HTSRTGYQPWVLCRESSGQIATAGPELMQQAGQVLDSIERRQEKARRLEGIEAGPKPSYL RDAVDDYRSEMAGLIKRYGDDLSRCDFIAAMKLASNGREPDEIAKAMAEASPAIMDRKAG HEADYIQRTLQKVMELPQVQEARAELAKQAEKQQYQGPSCH

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	782
Plasmid name	pCHE-A
GenBank accession number	NC_012006.1
Incompatibility group	IncQ1
Genome size	7560 bp
Coordinate of oriT [Strand]	5063..5150 [-]
Drug resistance	beta-lactamase GES-5
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterobacter cloacae CHE-2 [550]

Reference

[1] Poirel L et al (2009) Integron mobilization unit as a source of mobility of antibiotic resistance genes. Antimicrob Agents Chemother. 53(6):2492-8. [PMID:19332679]