oriTDB
The information of the oriT region
oriTDB accession number 100290
Name oriT_pXFAS01  insolico
Sequence Completeness intact
oriT length 103 nt
oriT sequence AGCCTCGCAGAGCAGGATTCCCGTTGAGTACCGAAGGTGCGAATAAGGGAAAGTGAAGAA GGAACACCGGATTTCCGGTGGGCCTACTTCACATATCCTGCCC
IR (inverted repeat)[53-70] [75-92] nt  (GTGAAGAAGGAACACCGG..CCGGTGGGCCTACTTCAC)
Location of nic site [100-101] nt
Conserved sequence flanking the
  nic site 		TATCCTG|C
Note predicted by the oriTfinder
L:53-70;R:75-92;D:100-101
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 361
Name TrbI_pXFAS01 insolico
GenBank accession number WP_004086668
Family MOBP
Length 830 aa
UniProt ID B2IAU3
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.60E-53, Aligned region: 12..252
Note putative relaxase
Protein sequence [Download] MIAKHVPMRVVKKSDFRELVKYLSNQQGKQERVGCVTVTNCFQNNVLDAALEVQATQALN
TRSEADKTYHLLISFREGENPAPEILEVIESRVCAALGYADYQRVSVVHHDTDNLHIHVA
INKIHPKSYTIHTPYNDYKTLGEICKKLEREYGLEADNHTVRKTVGENRADDMERNAGVE
SLLGWIKRECTDQIKQVQSWSELHAVMQRNGLEIRERGNGLVITNGVGRSVKASSVARDF
SKAKLEARFGAFESFVKQAPSVSSVHARRIQAPLVEKVGRRPPPRSKGRTPSLSSVGVLH
VNSGIRYEQRPIYVKQASTAELYAMYKLEQQDLGAVRNVAIARARTKRDRKIEATKRLGR
IKRAAIKLMRGPGVNKKLLYALARKSLKEGVEKANTDYLKARDAAYATYHRRVWADWLQV
QATQGNAEALAALRAREMRQWWSCNIFGGLQARRTGPVPGLKPDNITKAGTIIYRVGSTA
IRDDGDLLNVSHGAGDYGVEAALRMAMYRYGERITVKGSDEFKKRVVQIAAAARLNISFD
DEVLDKRRKQLVSDAAVIVKLTDAMLSDKTAQFTQAQASRVGAFQEDALSEYDAAISRSI
NLANQEQINEQQRTRAKHYFVQRWSDRGGPNSRRDGHATRIRSGGGGSGKHGASGATVIS
SAGGFNKPNVGGIGAKPPPASTHRLRNLHQLGVVQFSRGSEVLLPRHVSDCVGEQQTERN
HRVRRDVHTAGITSSELMAVDKYIAERELKRVREFDIMKNRRYNEDDAGMVKFAGLRQVD
GKSLVLLKRNDEVIVLAIDVATARRMKRFSLGDEVLLTKKGTLKTKGRNR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 752
Plasmid name pXFAS01
GenBank accession number NC_010579.1
Genome size 38297 bp
Coordinate of oriT  [Strand] 26824..26926 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Xylella fastidiosa M23 [405441]
Reference
[1] Chen J et al (2010) Whole genome sequences of two Xylella fastidiosa strains (M12 and M23) causing almond leaf scorch disease in California. J Bacteriol. 192(17):4534. [PMID:20601474]

Microbial Bioinformatics Group in MML, SJTU