oriTDB
The information of the oriT region
oriTDB accession number 100269
Name oriT_pO86A1  insolico
Sequence Completeness intact
oriT length 542 nt
oriT sequence TTACTCTGGCCATAAGATAAAGCCTTTCATTATTAAGCAATGAACTTTTCACTATAAATA TGCATATAGTGTTTACAAGTAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTAT AAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAA TTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTC TCTATTTAATAATTCAGAATTATTAAATATAAATAGCGTTTGTTAATTACATGATTTAAA ACGCAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCA CCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAG GTGGAAAAATGAAAAAATGGATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCC AGGCCACTGATTGCTTTGATCTTGCAGGCCGGGATTACAAAATAGATCCGGATTTACTGA GA
IR (inverted repeat)[243-255] [257-269] nt  (TATTTAATAATTC..GAATTATTAAATA)
[311-318] [321-328] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [337-338] nt
Conserved sequence flanking the
  nic site 		GTAGTGTGT|GG
Note predicted by the oriTfinder
L:243-255;R:257-269;L:311-318;R:321-328;D:337-338
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 340
Name TraI_pO86A1 insolico
GenBank accession number YP_788091
Family MOBF
Length 1756 aa
UniProt ID Q08JE3
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 3.90E-76, Aligned region: 10..284
TraI [PF07057.10], Evalue: 9.10E-55, Aligned region: 1434..1556
AAA_30 [PF13604.5], Evalue: 1.90E-34, Aligned region: 968..1160
AAA_19 [PF13245.5], Evalue: 1.80E-08, Aligned region: 973..1108
Note putative relaxase
Protein sequence [Download] MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRL
PDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEAL
ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKV
GKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVVGKHGMWEMPGVPVEAFSSRSQA
IREAVGEGASLKSRDVAALDTRKSKQHVDPEIRMAEWMQTLKETGFDIRAYRDAADQRAE
IRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARVG
IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE
RLSGELITGRRQLQDGMTFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQILITDSGQR
TGTGSALMSMKEAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ
VSGVREQAILTQSIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN
PETRSHDRYVIDRVTAQSHSLTLRNAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV
TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGH
SVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFNMVDLLTEAKSFAAEGTG
CTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMER
VPGELMETLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRV
VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM
ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAV
YSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGET
FPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLEDAEGNTRLIS
PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT
RVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMK
QHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAV
LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDCNGRSAGIWLNPLTTDDGNGLRGFSGE
GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT
GGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREVARENLLQERLQQME
RDMVHDLQKEKTLGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 731
Plasmid name pO86A1
GenBank accession number NC_008460.1
Incompatibility group IncFIB(AP001918)
Genome size 120730 bp
Coordinate of oriT  [Strand] 64739..65280 [-]
Drug resistance insolico resistance to neomycin, kanamycin, bleomycin, and streptomycin
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli DIJ1 [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU