oriTDB
The information of the oriT region
oriTDB accession number 100268
Name oriT_pBP136  insolico
Sequence Completeness intact
oriT length 373 nt
oriT sequence CTCGCCTCGTCGTCTCTCTGCCGGCGGTAGCCGGCTGCCTCGCAGAGCAAGATGCCCCGT TGAGTGCCACCGGCGCGAATAAGGGGAAGCGAAGATAGATAACCGGCTTGCCGGTTAGCT AACTTCGCCCATCTTGCCCGCCTTACGGCGTTAATAACGCCAAGGAAAGTCTACACCAGC CACTACGATTTATCCGCAACTATCGCGCTATCAGGCCGCAAAAGCAGCAACGGATATAGC GAAACCTGCCACAATGGCCCATAATGCGGCTGTCGAAGCGTGCCAATGCACGCCGATAGC GGGCTTTTTGCGTTTCCGTAGCGCCGCTTAGTAGCGTTACATTTGCGACGAGAGGATTAG ATGGACGAACACG
IR (inverted repeat)IR1: [64-68] [72-76] nt  (GTGCC..GGCGC)
IR2: [89-107] [110-128] nt  (GCGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCGC)
Location of nic site [136-137] nt
Conserved sequence flanking the
  nic site 		CATCTTG|C
Note predicted by the oriTfinder
L:64-68;R:72-76;L:89-107;R:110-128;D:136-137
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 339
Name TraI_pBP136 insolico
GenBank accession number YP_787961
Family MOBP
Length 745 aa
UniProt ID Q08KX8
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 4.20E-51, Aligned region: 12..252
Note putative relaxase
Protein sequence [Download] MILKHVPMRSLGKSDFAELANYITDAQSKEHRLGEVRLTNCHAATVGDAITEVLATQHTN
TRATGDKTYHLLVSFPPGENPPAAVLQQVEERVCAALGYSEHQRISAVHNDTDHLHIHIA
INKIHPVRNTMHEPFQAYRTMADIAVKLEKEFGLQQVNHSSKRTLAEGRAGDMEHHAGVE
SLVGWIRRECLDEITNAQTWQELHQVMRENGLELRSRANGFVIEASDGTMVKASTVARDL
SRPSLEARLGPFEASPERQAQTTAKRQYRKDPIRLRVNTVELYAKYRADQQSLTTARGQA
LERARHRKNRLIEAAKRSHRLRRATIKVVGEGRTTKKLLYAQASKALRSEIQAINKQFQQ
ERAALYAEHSRRTWVDWLKKEAHHGNTEALAALRAREKAGLKGNTIRGEGQAKPGHAPAV
DNITKKGTIIFRAGMSAVRDDGDRLQVSREATREGLQEALRLAMQRYGNRITVNGTVEFK
AQMIRAAVDSQLPITFTDPALESRRQALLNKENTHERTERPEHRGRTGRGAGGPGQWPLA
DQHATGAAAVARAGDGRPAAGRGDRADAGIHAANVHRKPDVGRLGRKPPPQSQHRLRALS
ELGVVRIAGGSEVLLPRDVPRHVEQQGTQPDHALRRAISRPGTGVGQTPPGVAAADKYIA
EREAKRLKGFDIPKHSRYTAGDGALTFQGTRTIEGQALALLKRGDEVMVMPIDQATARRL
TRIAVGDAVSITAKGSIKTSKGRSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 730
Plasmid name pBP136
GenBank accession number NC_008459.1
Incompatibility group IncP(Beta)
Genome size 41268 bp
Coordinate of oriT  [Strand] 39329..39701 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Bordetella pertussis BP136 [520]
Reference
[1] Kamachi K et al (2006) Plasmid pBP136 from Bordetella pertussis represents an ancestral form of IncP-1beta plasmids without accessory mobile elements. Microbiology. 152(Pt 12):3477-84. [PMID:17159199]

Microbial Bioinformatics Group in MML, SJTU