oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100255
Name	oriT_pC221
Sequence Completeness	core
oriT length	53 nt
oriT sequence	ATGGGATATAATCCCATCAAGTACGGCGTGCTAGAACGAAGTGGATAGCAAAGCCAGTGC TTGCCAAA
IR (inverted repeat)	[12-19] [35-42] nt (GTATATTC..GAATATAC)
Location of nic site	[50-51] nt
Conserved sequence flanking the nic site	CGCTTG\|CCA
Note	predicted by the oriTfinder
	L:12-19;R:35-42;D:50-51

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	326
Name	pC221_004_pC221
GenBank accession number	YP_232728
Family	MOB_P
Length	315 aa
UniProt ID	A9CQY2
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 5.20E-79, Aligned region: 8..239
Note	putative relaxase
Protein sequence [Download]	MATTKLGNTKSASRAINYAEKRAEEKSGLNCDVDYAKSAFKQTRALYGKEDGIQAHTVIQ SFKPGEVTPEQCNQLGLELAEKIAPNHQVAVYTHTDKDHYHNHIVINSVDLETGKKYQSN KKQRDLVKKENDNICREHGLSVTERGIAKMRYTQAEKGIVFDRDEYSWKDELRDLIENAK THTSNLETFSEHLEEKGVGVKLRGETISYKPENENKWVRGRTLGSEYEKGAIDHEHERHQ KQQREPEYADEFKINWDAVEQHTEQLKQRRVERAQETKQAHSKISSRDTRESENQRERAK GNNIRIERGDEGLSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	717
Plasmid name	pC221
GenBank accession number	NC_006977.1
Genome size	4555 bp
Coordinate of oriT [Strand]	3101..3153 [+]
Drug resistance	chloramphenicol resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus aureus [1280]

Reference

[1] Brenner DG et al (1985) The use of synthetic oligonucleotides with universal templates for rapid DNA sequencing: results with staphylococcal replicon pC221. EMBO J. 4(2):561-8. [PMID:3860383]

[2] Shaw WV et al (1985) Chloramphenicol acetyltransferase gene of staphylococcal plasmid pC221 Nucleotide sequence analysis and expression studies. FEBS Lett. 179(1):101-6. [PMID:3855295]