oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100253
Name	oriT_pCF10
Sequence Completeness	intact
oriT length	215 nt
oriT sequence	AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA AAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)	[61-76] [80-95] nt (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT) [123-134] [139-150] nt (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site	[135-136] nt
Conserved sequence flanking the nic site	TGG\|T
Note	predicted by the oriTfinder
	L:61-76;R:80-95;L:123-134;R:139-150;D:135-136

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	324
Name	PcfG_pCF10
GenBank accession number	YP_195793
Family	MOB_P
Length	561 aa
UniProt ID	Q7X5P3
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.10E-57, Aligned region: 13..300
Note	putative relaxase
Protein sequence [Download]	MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSG HGIVDPTNPYEEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSF SPEDDLTPEKIHEIGYNTVMELTGGKYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQ KGTKRVFEQISDKIAAKEGAKIIEKSPKQTHTKYTKWQTESIYKQKIKSRLDFLLEQSSS INDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSLSKSDPTRYNYEKIIERLKEN KNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYGYGNSGKLFVG GYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLL ENELQGDPELIQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQL HEIQAVQEKEPEKQTDIKPKL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	715
Plasmid name	pCF10
GenBank accession number	NC_006827.2
Genome size	67673 bp
Coordinate of oriT [Strand]	55445..55659 [-]
Drug resistance	tetracycline resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterococcus faecalis [1351]

Reference

[1] Hirt H et al (2005) Characterization of the pheromone response of the Enterococcus faecalis conjugative plasmid pCF10: complete sequence and comparative analysis of the transcriptional and phenotypic responses of pCF10-containing cells to pheromone induction. J Bacteriol. 187(3):1044-54. [PMID:15659682]

[2] Hedberg PJ et al (1996) Identification and characterization of the genes of Enterococcus faecalis plasmid pCF10 involved in replication and in negative control of pheromone-inducible conjugation. Plasmid. 35(1):46-57. [PMID:8693026]

[3] Nakayama J et al (1994) The prgQ gene of the Enterococcus faecalis tetracycline resistance plasmid pCF10 encodes a peptide inhibitor, iCF10. J Bacteriol. 176(23):7405-8. [PMID:7545961]

[4] Ruhfel RE et al (1993) Cloning and characterization of a region of the Enterococcus faecalis conjugative plasmid, pCF10, encoding a sex pheromone-binding function. J Bacteriol. 175(16):5253-9. [PMID:8349565]

[5] Kao SM et al (1991) Molecular and genetic analysis of a region of plasmid pCF10 containing positive control genes and structural genes encoding surface proteins involved in pheromone-inducible conjugation in Enterococcus faecalis. J Bacteriol. 173(23):7650-64. [PMID:1938961]