 oriTDB accession number | 100224 |
| Name | oriT_Ti  |
Sequence Completeness  | core |
| oriT length | 37 nt |
| oriT sequence | CCAAGGGCGCAATTATACGTCGTGATACGACGCGTTG |
| IR (inverted repeat) | [18-23] [27-32] nt (CGTCGT..ACGACG) |
| Location of nic site | [7-8] nt |
| Conserved sequence flanking the nic site | _ |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 295 |
| Name | TraA_Ti |
| GenBank accession number | NP_059695 |
| Family | MOBQ |
| Length | 1100 aa |
| UniProt ID | Q44363 |
| PDB ID | _ |
| Pfam | MobA_MobL [PF03389.14], Evalue: 1.80E-74, Aligned region: 17..243 AAA_30 [PF13604.5], Evalue: 5.10E-56, Aligned region: 381..569 Viral_helicase1 [PF01443.17], Evalue: 8.30E-05, Aligned region: 661..737 AAA_19 [PF13245.5], Evalue: 3.40E-09, Aligned region: 386..513 UvrD_C_2 [PF13538.5], Evalue: 1.90E-06, Aligned region: 692..736 |
| Note | putative relaxase |
| Protein sequence [Download] | MAIAHFSASIVSRGSGRSVVLSAAYRHCAKMEYEREARTIDYTRKQGLLHEEFVLPADAP KWVRSLIADRSVSGASEAFWNKVEAFEKRADAQLARDLTIALPLELSAEQNIALVRDFVE NHILAKGMVADWVYHENPGNPHIHLMTTLRPLSDESFGSKKVAVIGEDGQPVRTKSGKIL YELWAGSTDDFNVLRDGWFERLNHHLALGGIDLKIDGRSYEKQGINLEPTIHLGVGAKAI ERKAEQRGVRPELERVELNEQRRSENTRRILNNPAIVLDLITREKSVFDERDVAKVLHRY IDDPALFQQLMIKIILNRQVLRLQRETIDFSTGEKLPARYSTRAMIRLEATMARQATWLS NREGRGVSPTALDATFRRHERLSDEQKAAIEHVAGPARIAAVVGRAGAGKTTMMKAAREA WELAGYHVVGGALAGKAAEGLEKEAGIQSRTLASWELRWKRGRDLLDDKTIFIMDEAGMV ASKQMAGFVDTAVRAGAKIVLVGDPEQLQPIEAGAAFRAIADRIGYAELETIYRQREEWM RKASLDLARGNVENALSAYRANVRITGERLKAEAVERLIADWNHDYDQTKTILILAHLRR DVRMLNVMAREKLVERGMVGEGHLFRTADGERRFDAGDQIVFLKNEGSLGLKNGMIGHVV VAAANRIVATVGEGDQRRQVIVEQRFYNNLDHGYATTIHKSQGATVDRVKVLASLSLDRH LTYVAMTRHREDLQLYYGTRSFSFNGGLAKVLSRRQAKETTLDYEHGQFYREALRFAETR GLHVVQVARTMVRDRLDWTLRQKAKLVDLSRRLAAFAAHLGITQSPKTQTMKETAPMVAG IKTFSGSVSDIVGDRLGTDPSLKRQWEEVSARFAYVFADPETAFRAMNFAALLADKEVAK QILQKLEAEPGSIGPLKGKTGILASKPEREARRVAEVNVPALKRDLEQYLRMRETVTQRL QTDEQSLRQRVSIDIPALSPAARVVRERVRDAIDRNDLPAAIAYALSNRETKLEIDGFNQ AVTERFGERTLLSNAAREPSGKLYEKLSEGMKPEQKEELKQAWPVMRTAQQLVAHERTVH SLKLAEEHRLTQRQTPVLKQ |
Reference| ID | 686 |
| Plasmid name | Ti |
| GenBank accession number | NC_002377.1 |
| Genome size | 194140 bp |
| Coordinate of oriT [Strand] | 30720..30756 [+] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Agrobacterium tumefaciens [358] |
Reference| [1] Lyi SM et al (1999) Mannopinic acid and agropinic acid catabolism region of the octopine-type Ti plasmid pTi15955. Mol Microbiol. 31(1):339-47. [PMID:9987134] |
| [2] Kalogeraki VS et al (1998) Wound-released chemical signals may elicit multiple responses from an Agrobacterium tumefaciens strain containing an octopine-type Ti plasmid. J Bacteriol. 180(21):5660-7. [PMID:9791116] |
| [3] Oger P et al (1998) Octopine-type Ti plasmids code for a mannopine-inducible dominant-negative allele of traR, the quorum-sensing activator that regulates Ti plasmid conjugal transfer. Mol Microbiol. 27(2):277-88. [PMID:9484884] |
| [4] Alt-Mörbe J et al (1996) The conjugal transfer system of Agrobacterium tumefaciens octopine-type Ti plasmids is closely related to the transfer system of an IncP plasmid and distantly related to Ti plasmid vir genes. J Bacteriol. 178(14):4248-57. [PMID:8763954] |
| [5] Fuqua C et al (1996) Localization of OccR-activated and TraR-activated promoters that express two ABC-type permeases and the traR gene of Ti plasmid pTiR10. Mol Microbiol. 20(6):1199-210. [PMID:8809772] |
| [6] Kim KS et al (1996) Ti plasmid-encoded genes responsible for catabolism of the crown gall opine mannopine by Agrobacterium tumefaciens are homologs of the T-region genes responsible for synthesis of this opine by the plant tumor. J Bacteriol. 178(11):3275-84. [PMID:8655509] |
| [7] Valdivia RH et al (1991) Characterization of a putative periplasmic transport system for octopine accumulation encoded by Agrobacterium tumefaciens Ti plasmid pTiA6. J Bacteriol. 173(20):6398-405. [PMID:1655707] |
| [8] Habeeb LF et al (1991) Transcription of the octopine catabolism operon of the Agrobacterium tumor-inducing plasmid pTiA6 is activated by a LysR-type regulatory protein. Mol Plant Microbe Interact. 4(4):379-85. [PMID:1799699] |
| [9] Winans SC et al (1987) Characterization of the virE operon of the Agrobacterium Ti plasmid pTiA6. Nucleic Acids Res. 15(2):825-37. [PMID:3547330] |
| [10] Yanofsky MF et al (1986) The virD operon of Agrobacterium tumefaciens encodes a site-specific endonuclease. Cell. 47(3):471-7. [PMID:3021341] |
| [11] Yanofsky MF et al (1986) Molecular characterization of a host-range-determining locus from Agrobacterium tumefaciens. J Bacteriol. 168(1):244-50. [PMID:3759904] |
| [12] Barker RF et al (1983) Nucleotide sequence of the T-DNA region from theA grobacterium tumefaciens octopine Ti plasmid pTi15955. Plant Mol Biol. 2(6):335-50. [PMID:24318453] |