oriTDB
The information of the oriT region
oriTDB accession number 100214
Name oriT_pLS55  insolico
Sequence Completeness incomplete
oriT length 35 nt
oriT sequence CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
IR (inverted repeat)[6-9] [10-13] nt  (AGAA..TTCT)
Location of nic site [24-25] nt
Conserved sequence flanking the
  nic site
TACCGTG|TTAT
Note _
L:6-9;R:10-13;D:24-25
Visualization of oriT structure
Reference
[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]
I. Information of Relaxase
ID 207
Name Mob_pLS55 insolico
GenBank accession number YP_001691307
Family MOBV
Length 439 aa
UniProt ID B0YMX1
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 5.30E-37, Aligned region: 1..187
Note relaxase
Protein sequence [Download] MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDE
RLSEVYCMNRKDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSAN
VHNDETTPHMHFDFVPVVWDEKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQE
GILNDKTIGIENIHSLKKYSAEIEQQKEEMANEFKSFKYPQKVLKSIEREVEKKTVGVFN
KKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEIKNLKVNVKNFEEDNEHLRYR
NIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLHNLENDRMPKN
KEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL
SQQRQQQKKTKSRSSGMEL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 291 _ insolico
 N/A
Information of plasmid
ID 207
Plasmid name pLS55
GenBank accession number NC_010375.1
Genome size 5031 bp
Coordinate of oriT  [Strand] 2038..2072 [+]
Drug resistance insolico resistance to tetracycline (tetL)
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactobacillus sakei Rits9 [1599]
Reference
 N/A