 oriTDB accession number | 100212 |
| Name | oriT_R27  |
Sequence Completeness  | intact |
| oriT length | 285 nt |
| oriT sequence | GGTTATTGCTACTTAATGCCGATAACGACTCAGGCTTTGAGGTTTTTTTATACGGTTCAC ATTTCGTTAGCAAGGTCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGT TACAAGTAAGGGTAATGGCTGAAAGGTTAGTTTTAAGGTTCAAAGCGGCAGTATTAAAAT TCCAAAAGTTACTTTTCATCCTTCAGAATCCAGACCTTAATTTCATGTAGAAGATTCGTA CAATTGTATTGGCGCAAGGACAATCCGCACATGTCAGAATCAGAT |
| IR (inverted repeat) | [189-194] [196-201] nt (AAAAGT..ACTTTT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site | _ |
| Note | _ |
Visualization of oriT structure (The oriT was characterized experimentally) Reference| [1] Lawley TD et al (2002) Functional and mutational analysis of conjugative transfer region 1 (Tra1) from the IncHI1 plasmid R27. J Bacteriol. 184(8):2173-80. [PMID:11914349] |
| ID | 205 |
| Name | TraI_R27  |
| GenBank accession number | NP_058333 |
| Family | MOBH |
| Length | 1011 aa |
| UniProt ID | Q9L5M2 |
| PDB ID | _ |
| Pfam | TraI_2 [PF07514.10], Evalue: 4.30E-35, Aligned region: 99..398 |
| Note | - |
| Protein sequence [Download] | MNFRALFLSMQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGEQKYTVPDHPNPEVLKF IEYPTRPAGIQTFNEQSILSLYRDKLHSISMMLAISDGDIREDAYTFTNLVLKPLIEYIR WIHLLPASENHHHNGIGGLLSHSLEVAMISLKNANHSELRPIGYQDEEVVRRKVYLYAAF ICGLVHDAGKVYDLDIVSLNLSETLTWAPSSQSLLDWARENNVVEYEIHWRKRIHNQHNI WSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDGNDFLSKCVRTSDYYSTGTDL NVLRDPIMGLRSNDAAARAIGTIKHNFTSININNYKTKPMHIIIVNGEVYLNENAFLDFV LSDFAAHKFNFPQGDAGKTVLVESLVQRGYVEPYDDERVVHYFIPGTYSENEIASIFRNG IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRE SVTRVTDTVNDAIENTPQYGLQLVNGPDADSNNIISSENTESITDSLEESGADISNEIFE TQVVTAIDTAETVNADEPEQVEEHDDRSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLD LDAAIALIPGIDEAAFCNGPFFQLTYRDGSLDGMWIVRDVNNLRLIQLGDNCAGMQVSTS EPRNTSSLKSLFDTSMYQPLDIPEAPSVNEAASPPQTPLELPQPRLNAPVAEEASSVAEQ TNAHSEPDSVIATEYEQYGHLLEETLDSDGEAYSDLIASDSTEAEYPATDPQSSDFAQLP RETALSVAPGDLDYSEGAIKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALA RLFAVSTHAEKKHEKTQEPSPVKEVKNPTSSTTVKAPISIEPPGAEEKEAVEEFTLLNDG EVTELEYVEIATMLHQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPDL FNQLPQYQVTSGAVVNTKCIAFNIPTLVAASDRAKVDIELIINKLKEVGNL |
Reference| [1] Lawley TD et al (2002) Functional and mutational analysis of conjugative transfer region 1 (Tra1) from the IncHI1 plasmid R27. J Bacteriol. 184(8):2173-80. [PMID:11914349] |
| ID | 205 |
| Name | TraG_R27 |
| SecReT4 accesion number | _ |
| GenBank accession number | NP_058332 |
| Family | TrwB/TraD |
| Length | 694 |
| UniProt ID | Q9L5M3 |
| PDB ID | _ |
| Pfam | TrwB_AAD_bind [PF10412.8], Evalue: 8.50E-10, Aligned region: 396..648 TraG-D_C [PF12696.6], Evalue: 1.30E-08, Aligned region: 504..632 AAA_10 [PF12846.6], Evalue: 1.20E-06, Aligned region: 194..304 |
| Note | _ |
| Protein sequence [Download] | MTKSKRTNLHAQENFYRPILEYRSASVLLICAVIMLVMGFRSDGVNIAPIILYTAVFLLL VCLYRCKTAHPYLMAHWRVFQRQIMFISLKSLRTINKSNFFSNERKYRQLVQEYKKTNRP VPDRKTYFCNGFEWGPEHADRAYQIANLSSDKREIALPFVLSPIARHFETMARSMGGNNA IFAVDRRAPIFVTEDNWFGHTLITGNVGTGKTVLQRLLSISMLHLGHVIVVIDPKNDAEW RQSLMDEASELGLPFYKFHPAQPSSSVCIDVCNAYTNVSDLTSRLLSLVSVPGEVNPFVQ YAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMSVVNLTIKVMECCFARHYGPDVWMEKV KYASNDTLQVRFKRLTEWFNAHFLNYEGAEPIEWIDTVGRLVDYSMSDPEHMSKMTAGIM PLFSRLTEQPLNELLSPSPNTLTSREIVTSDGMFSTGGVLYISLDGLSNPESARAISQLI MSDLTSCAGSRYNADDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQT ISDFIAAANAETANRITGLCNNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETT LPHNNFSGSISERKQTTLEESIPKELLGQVPKFHIVARLQDGRKVVGQIPIAVSEKAMKP NTTLLEMFLKPAGKVTLRQNVGLSYLNKYLRKLH |
Reference| [1] Lawley TD et al (2002) Functional and mutational analysis of conjugative transfer region 1 (Tra1) from the IncHI1 plasmid R27. J Bacteriol. 184(8):2173-80. [PMID:11914349] |
| ID | 205 |
| Plasmid name | R27 |
| GenBank accession number | NC_002305.1 |
| Incompatibility group | IncHI1 |
| Genome size | 180461 bp |
| Coordinate of oriT [Strand] | 108637..108921 [+] |
| Drug resistance | tetracycline resistance |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Salmonella enterica subsp. enterica serovar Typhi (Salmonella typhi) [90370] |
Reference| [1] Lawley TD et al (2002) Functional and mutational analysis of conjugative transfer region 1 (Tra1) from the IncHI1 plasmid R27. J Bacteriol. 184(8):2173-80. [PMID:11914349] |