oriTDB
The information of the oriT region
oriTDB accession number 100206
Name oriT_CloDF13  experimental
Sequence Completeness incomplete
oriT length 13 nt
oriT sequence GTGGGTGGTCGGG
IR (inverted repeat)_
Location of nic site [7-8] nt
Conserved sequence flanking the
  nic site
_
Note CloDF13 familiy
D:7-8
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Francia MV et al (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]
[2] Núñez B et al (2001) Two atypical mobilization proteins are involved inplasmid CloDF13 relaxation. Mol Microbiol. 39(4):1088-99. [PMID:11251827]
I. Information of Relaxase
ID 199
Name MobC_CloDF13 experimental
GenBank accession number NP_052376
Family MOBC
Length 148 aa
UniProt ID P08097
PDB ID _
Pfam
Note relaxase
Protein sequence [Download] MILRGCTMALERYNVSQPKRQAERGENTADPALAAGRACSTAELVARRLGIAAVQPVYRF
LDTWWRKGCWSGEITRLTAVRSACGADAARGGVLVRRGRAADGHYSVSCRRGVCAQLPRT
GWPFELLRLAMEGNSRGAPAGGTCTVLP
Reference
[1] Francia MV et al (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]
[2] Núñez B et al (2001) Two atypical mobilization proteins are involved inplasmid CloDF13 relaxation. Mol Microbiol. 39(4):1088-99. [PMID:11251827]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 280 _ insolico
Information of T4CP
ID 199
Name MobB_CloDF13 experimental
SecReT4 accesion number _
GenBank accession number NP_052377
Family TrwB/TraD
Length 529
UniProt ID P08098
PDB ID _
Pfam
Note Type IV Secretion System Coupling Protein
Protein sequence [Download] MGLSHKERREYGRSYKRDNNRTAPFSCWPRWIRPGGWSGHGGMSGTTRTGVTARLAFWGG
WLSPCLSRAGEKPSCVRTSTRTQIITCCSRLTCSGLPGARAGWLSGLCWRGSGVLLLDGH
FAAADEAVNRKLNRRAGAQPATGEMTDVRQPAFRGAGTVNALARFFHGRGPETAGVFLGK
DEQGEPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRSVRDFGDAVVYFDQRGPVAAL
LPAHCPNFTLLDLRPGKPAQLNLFRDLDQYALKNLLVAGFNLSETGHVADHYRISEQKAA
KLIAEQVSAGGKHSAGAGGGVRAAGGPEKGREGADHQAGERGRPERPADGQRHRRGGDNQ
RRRLLYVIGSMDDEAVIRVQKMLFARCAQIIIARDEFRRWPHASIMLDEIKYLLSKYVLN
ALGTVRSRDCNLRLAHHAGRLRAAGQDLPADFVKTTVLDNTPIRWFYRAASQESRSGAGQ
TGEIRVDVERRGPAGRRGTWSISAGTASSRRSRPLFDVNTLQHLLTGSR

Reference
[1] Cabezón E et al (1994) Two atypical mobilization proteins are involved inplasmid CloDF13 relaxation. J Bacteriol. 176(14):4455-8. [PMID:8021231]
[2] Buscher BA et al (2005) Two atypical mobilization proteins are involved inplasmid CloDF13 relaxation. J Bacteriol. 187(9):2927-38. [PMID:15838018]
[3] Francia MV et al (2004) Two atypical mobilization proteins are involved inplasmid CloDF13 relaxation. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]
[4] Núñez B et al (2001) Two atypical mobilization proteins are involved inplasmid CloDF13 relaxation. Mol Microbiol. 39(4):1088-99. [PMID:11251827]
Information of plasmid
ID 199
Plasmid name CloDF13
GenBank accession number NC_002119.1
Genome size 9957 bp
Coordinate of oriT  [Strand] 9750..9762 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Enterobacter cloacae [550]
Reference
 N/A