oriTDB
The information of the oriT region
oriTDB accession number 100199
Name oriT_pKJ36  insolico
Sequence Completeness core
oriT length 32 nt
oriT sequence ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT
IR (inverted repeat)[1-8] [12-19] nt  (ATGCAACC..GGTTGCAT)
Location of nic site [28-29] nt
Conserved sequence flanking the
  nic site
TAAGTGCG|CCCT
Note _
L:1-8;R:12-19;D:28-29
Visualization of oriT structure
Reference
[1] Francia MV et al (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]
[2] Becker EC et al (2003) Relaxed specificity of the R1162 nickase: a model for evolution of a system for conjugative mobilization of plasmids. J Bacteriol. 185(12):3538-46. [PMID:12775691]
I. Information of Relaxase
ID 192
Name MobB_pKJ36 insolico
GenBank accession number NP_072178
Family MOBQ
Length 346 aa
UniProt ID Q9F152
PDB ID _
Pfam MobA_MobL [PF03389.14], Evalue: 3.50E-66, Aligned region: 17..254
Note MobA/MobL family; 39.0 kDa; pI=10.66; similar to plasmid pKJ50 MobA protein
Protein sequence [Download] MAIYHLSVSNVSRASGSRATATLSYITGKRVHDERRGETYDYGRKERVLRVGTLLPEGAP
AEFADPAVLFNAVELHETGRTARPAKKIVVALPREFTPRQRVQALEEYIRENLNADGYAA
TYAIHEDREGNNPHAHILVANRQIDPATGGWARLKQRMEYVLDERGERVPLIDPETGRQK
TDKRGRRQWKRTSVSLNPLDRKAKLKALRESWAKTCNARLDETARIDHRSLEDQGSDLEP
TIHEGYAARAIERAGGVSERCEANREIRRSNGLLTAIRTELGRIFDRLGELFAAKIRQLR
QRQAERNRPRSRTGATSRATRAASSKPTGPTTRQRSVENSWEPRPT
Reference
[1] Francia MV et al (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 272 _ insolico _ _ _
 N/A
Information of plasmid
ID 192
Plasmid name pKJ36
GenBank accession number NC_002635.1
Genome size 3625 bp
Coordinate of oriT  [Strand] 3482..3513 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Bifidobacterium longum KJ [216816]
Reference
 N/A