oriTDB
The information of the oriT region
oriTDB accession number 100196
Name oriT_pKJ50  insolico
Sequence Completeness core
oriT length 35 nt
oriT sequence TGAATGTTACCACCGGTAACATGTAAGTGCGCCCT
IR (inverted repeat)[4-11] [15-22] nt  (ATGTTACC..GGTAACAT)
Location of nic site [31-32] nt
Conserved sequence flanking the
  nic site 		TAAGTGCG|CCCT
Note _
L:4-11;R:15-22;D:31-32
Visualization of oriT structure
Reference
[1] Park MS et al (1999) Sequence analysis of plasmid pKJ50 from Bifidobacterium longum. Microbiology. 145 ( Pt 3):585-92. [PMID:10217492]
[2] Parker C et al (2005) Elements in the co-evolution of relaxases and their origins of transfer. Plasmid . 53(2):113-8. [PMID:15737398]
[3] Becker EC et al (2003) Relaxed specificity of the R1162 nickase: a model for evolution of a system for conjugative mobilization of plasmids. J Bacteriol. 185(12):3538-46. [PMID:12775691]
I. Information of Relaxase
ID 189
Name MobA_pKJ50 insolico
GenBank accession number AAD00257
Family MOBQ
Length 345 aa
UniProt ID Q9ZA19
PDB ID _
Pfam MobA_MobL [PF03389.14], Evalue: 7.80E-34, Aligned region: 60..224
Note relaxase
Protein sequence [Download] MRDERTGEAFNGFGRRSASSMYARCCPRARPASTSTRSVCSTPSRWPRNVPTRGPRRRSW
SPCPASSTPANAFRALEDFISWNITANGYACTYAIHTDKDGRNPHAHILVANRRIDPKTG
RWAAKSRSEFALDANGRRIPVIDPDTGRQKIGARNRKVWKRVNVSNNPLDSKEFLERLRR
EWADSCNALLPGYAVIDHRSFKARGIERIPTIHEGYASREMEKRGGRGDLCEENRRIQAL
NRLLDALRAMIGRLSDQAQGILTAVKRRQRPSGAPQAPSSPEPAERPQERPQARETPPQA
PSSPEPAERPQERPQARETPATGPAGTPEAHQDEKGAHRQVQDTA
Reference
[1] Parker C et al (2005) Elements in the co-evolution of relaxases and their origins of transfer. Plasmid . 53(2):113-8. [PMID:15737398]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 269 _ insolico _ _ _
 N/A
Information of plasmid
ID 189
Plasmid name pKJ50
GenBank accession number NC_004978.1
Genome size 4960 bp
Coordinate of oriT  [Strand] 2462..2496 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Bifidobacterium longum KJ [216816]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU