oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100190
Name	oriT_pIP1714
Sequence Completeness	core
oriT length	38 nt
oriT sequence	CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC
IR (inverted repeat)	[2-8] [13-19] nt (ACTTTAT..ATAAAGT)
Location of nic site	[27-28] nt
Conserved sequence flanking the nic site	TAGTGTG\|TTAT
Note	_
	L:2-8;R:13-19;D:27-28

Visualization of oriT structure

Reference

[1] Grohmann E et al (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]

I. Information of Relaxase

ID	183
Name	Mob/Pre_pIP1714
GenBank accession number	AAC61672
Family	MOB_V
Length	420 aa
UniProt ID	O87277
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 1.70E-73, Aligned region: 1..193
Note	relaxase
Protein sequence [Download]	MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKE IIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIA YATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERG SDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPT GEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYK KLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLK ERTDRLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	261	_	_	_	_

N/A

Information of plasmid

ID	183
Plasmid name	pIP1714
GenBank accession number	AF015628.1
Genome size	4978 bp
Coordinate of oriT [Strand]	2356..2393 [+]
Drug resistance	resistance to streptogramins A and B
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus cohnii BM10711 [29382]

Reference

[1] Allignet J et al (1998) Characterization of a staphylococcal plasmid related to pUB110 and carrying two novel genes, vatC and vgbB, encoding resistance to streptogramins A and B and similar antibiotics. Antimicrob Agents Chemother. 42(7):1794-8. [PMID:9661023]