 oriTDB accession number | 100181 |
| Name | oriT_ColE1  |
Sequence Completeness  | core |
| oriT length | 60 nt |
| oriT sequence | GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA |
| IR (inverted repeat) | [3-12] [16-25] nt (GTGTCGGGGC..GCCCTGACCC) |
| Location of nic site | [56-57] nt |
| Conserved sequence flanking the nic site | CTGG|CTTA |
| Note | _ |
Visualization of oriT structure (The oriT was characterized experimentally) Reference| [1] Varsaki A et al (2009) Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins. J Bacteriol. 191(5):1446-55. [PMID:19114496] |
| [2] Francia MV et al (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531] |
| [3] Rogge ML et al (2013) Comparison of Vietnamese and US isolates of Edwardsiella ictaluri. Dis Aquat Organ. 106(1):17-29. [PMID:24062549] |
| ID | 174 |
| Name | MbeA_ColE1 |
| GenBank accession number | CAA33883 |
| Family | MOBP |
| Length | 517 aa |
| UniProt ID | P13658 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 7.10E-20, Aligned region: 49..247 |
| Note | relaxase |
| Protein sequence [Download] | MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDA LYHAGEIKGRQDVIQALTEAGLEVVRVTRTSISIADPNGGKNIRLKGAFYEQSFADGRGV REKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRRYSPVHSLDRGIAGKTPGRGE RGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRNQEAGRDIAET EREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREL EIREERLIQEREHVLSLERERQPEIQERTLDGPSLGW |
Reference| [1] Varsaki A et al (2009) Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins. J Bacteriol. 191(5):1446-55. [PMID:19114496] |
| [2] Francia MV et al (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 250 | MbeB_ColE1 | CAA33884 | 172 aa | _ |
| 2 | 251 | MbeC_ColE1 | CAA33882 | 107 aa | _ |
| ID | 174 |
| Plasmid name | ColE1 |
| GenBank accession number | NC_001371.1 |
| Incompatibility group | ColRNAI |
| Genome size | 6646 bp |
| Coordinate of oriT [Strand] | 1462..1521 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli [562] |
Reference