 oriTDB accession number | 100179 |
| Name | oriT_pC221  |
Sequence Completeness  | core |
| oriT length | 53 nt |
| oriT sequence | CCATCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACAACGCTTGCCA |
| IR (inverted repeat) | [12-19] [35-42] nt (GTATATTC..GAATATAC) |
| Location of nic site | [50-51] nt |
| Conserved sequence flanking the nic site | CGCTTG|CCA |
| Note | _ |
Visualization of oriT structure Reference| [1] Caryl JA et al (2006) Investigating the basis of substrate recognition in the pC221 relaxosome. Mol Microbiol . 60(5):1302-18. [PMID:16689804] |
| [2] Varsaki A et al (2009) Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins. J Bacteriol. 191(5):1446-55. [PMID:19114496] |
| ID | 172 |
| Name | OrfA_pC221 |
| GenBank accession number | NP_052695 |
| Family | MOBP |
| Length | 315 aa |
| UniProt ID | P03865 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 5.20E-79, Aligned region: 8..239 |
| Note | relaxase |
| Protein sequence [Download] | MATTKLGNTKSASRAINYAEKRAEEKSGLNCDVDYAKSAFKQTRALYGKEDGIQAHTVIQ SFKPGEVTPEQCNQLGLELAEKIAPNHQVAVYTHTDKDHYHNHIVINSVDLETGKKYQSN KKQRDLVKKENDNICREHGLSVTERGIAKMRYTQAEKGIVFDRDEYSWKDELRDLIENAK THTSNLETFSEHLEEKGVGVKLRGETISYKPENENKWVRGRTLGSEYEKGAIDHEHERHQ KQQREPEYADEFKINWDAVEQHTEQLKQRRVERAQETKQAHSKISSRDTRESENQRERAK GNNIRIERGDEGLSR |
Reference| [1] Caryl JA et al (2006) Investigating the basis of substrate recognition in the pC221 relaxosome. Mol Microbiol . 60(5):1302-18. [PMID:16689804] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 248 | _ | _ | _ |
| ID | 172 |
| Plasmid name | pC221 |
| GenBank accession number | NC_006977.1 |
| Genome size | 4555 bp |
| Coordinate of oriT [Strand] | 3101..3153 [+] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Staphylococcus aureus [1280] |
Reference