 oriTDB accession number | 100177 |
| Name | oriT_R721  |
Sequence Completeness  | core |
| oriT length | 53 nt |
| oriT sequence | CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT |
| IR (inverted repeat) | [5-21] [25-42] nt (CGATTGTAACATGACCG..CGGTCTTGTGTACAATCG) |
| Location of nic site | [50-51] nt |
| Conserved sequence flanking the nic site | TATCGTG|C |
| Note | _ |
Visualization of oriT structure (The oriT was characterized experimentally) Reference| [1] Varsaki A et al (2009) Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins. J Bacteriol. 191(5):1446-55. [PMID:19114496] |
| [2] Kim SR et al (1992) Nucleotide sequence of the R721 shufflon. J Bacteriol. 174(21):7053-8. [PMID:1400257] |
| ID | 170 |
| Name | NikB_R721  |
| GenBank accession number | NP_065331 |
| Family | MOBP |
| Length | 1272 aa |
| UniProt ID | Q9F553 |
| PDB ID | _ |
| Pfam | |
| Note | relaxase |
| Protein sequence [Download] | MIVRYGGGNDGIVDYLINGRKAERQYTRDELDHRVVLDGDLQTTDKIIDSIENKSQERYL HITLSFHESHVSNEVLKAVVDDYKKLLMNAYHPDEYSFYAEAHLPKIRHIQDNSTGELVE RKPHIHIVIPKVNLITEKFLNPVGDVTKGHTIEQLDAIQEFINNKYNLDSPKDYPRKDAD YGKIISRVKGDLYKEHHSELKGELLSRIENEKIENYSVFKDIVAEYGELRIRNAGKTNEY LAVKLPGDKKYTNLKSPLFRQNYIETRTLTLEKPTHKEIEKRLNAWLNKTSHEIKHIFNQ AEKTRELYKTLSPSQQIDFLQERIKEYDSREKLNERNSQQTSGRAGGYKSCPKKFARIRQ SEATVGLSRMPQRGMVYGINGFTRPDSVSVLSDISQRDLAKQLPQREHPGQDVRRDYDRQ FTESGIKSLERSSFLCETMFQTLNEAAEKNEITTMAEIRRNIDPVRFLSSAAERFNIIPA QHKIRTAKDGSPRFSVGNRNMNASDFLTKHINLAWKDAKSFLLEVYSQQLENTPYTRYPT YRRLTHHEARERLNSLNLSEKTLRNTIRFERGKLYNDLREMRRELKLIPREQRDIAVGVI VYKKLTTLERLSELDTEGRHIIRQYHADWHKDKDEMKALERLKSYLNFDEINAISADEPE LSLQKAVDSQRRLEEAKKINTKLKDLVMDKQDSRIVYRDQESEKPVFTDKGNFVVAGKNP SKEEIGIMLEYSREKFGGVLKLTGSEDFKKMCAEVAAEQDMKIILRPEQYQQMMLELKAE LQGNKFEQVETQENSQESESRIEKGDALKEQATEQEQATEQAQATEQEQATEQAQATEQA QATEQAQATEQAQATEQAQATEQAQDTEQAQATEQAQATEQAQATEQAQIAEQATSSYDP GVITRANILDSQMLSKGTNGEFGYLKSLDSDENEIWEVLGHVPGDSDDIFSVASFDNEND AKEFCKIVNELGIDRTQALIQEQLSSQQEQAAVQETYQESPEEILASISANEHMISGLEN FLVKDRSQFSSCNGDIVVEAEITRGEGGLYHLAVAGKHGLERGDAVARVDVTEQQFAAIT GKTPSEVLTGDQTSARVPVITGIHFSTRAIENVNKLEQQKDYVYFSTHEGLNAEIKDFSS LKDAIEWGRVECELHDLNKRDTVIYRVESEHISQGIDAVMKNAERVERHEIEKAQGRDCT PEDGKILEAIDRFEDKFRGEGLKFEREKAESDLLNHGFTREMAEDALGKQFVQAREDHQE SQQQRDDSQDMH |
Reference| # | ID | Name | GenBank | Length | Note |
| 1 | 245 | NikA_R721 | NP_065330 | 113 aa | relaxosome component |
| ID | 170 |
| Plasmid name | R721 |
| GenBank accession number | NC_002525.1 |
| Incompatibility group | IncI2 |
| Genome size | 75582 bp |
| Coordinate of oriT [Strand] | 29007..29059 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli K-12 [83333] |
Reference