oriTDB
The information of the oriT region
oriTDB accession number 100173
Name oriT_pEC3  insolico
Sequence Completeness incomplete
oriT length 8 nt
oriT sequence CTGGCTTA
IR (inverted repeat)_
Location of nic site [4-5] nt
Conserved sequence flanking the
  nic site
CTGG|CTTA
Note _
D:4-5
Visualization of oriT structure
Reference
[1] Nomura N et al (1996) Genetic organization of a DNA-processing region required for mobilization of a non-self-transmissible plasmid, pEC3, isolated from Erwinia carotovora subsp. carotovora. Gene. 170(1):57-62. [PMID:8621089]
I. Information of Relaxase
ID 166
Name MobA_pEC3 experimental
GenBank accession number BAA08130
Family MOBP
Length 498 aa
UniProt ID Q47385
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.80E-16, Aligned region: 41..247
Note relaxase
Protein sequence [Download] MIVKFHPRGRGGGAGPVDYLLGKDRNRDGASVLQGKPEEVRELIDASPYAKKYTSGVLSF
AEQDLPPGQREKLMASFERILMPGLDKDQYSVLWVEHQDKGRLELNFLVPNTELLTGKRL
QPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSALPEAKQEAAQTITRGLLA
LASSGELKTRQDVTEAVESAGFEVLRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGL
RAEIESARAEYRRDAESRIQRAREVCQSGTERKREENQRRYPRPRPEAVFSHEPAHERDA
AHGQPDVADHRAGFRPADRVERGHSVVAGAADTRQLHDHPGAEEHAGDAVREEQRRATHD
LWRAETPLREGEPGSGNVRRGFELDDTGGEIAHDGAGKTVVERIRAATAGLLEKAGRVGE
RLRGMADDVWSYATGERSAERARHGLEQAGAEFERAAKPVVERLNVIEQRRQYEHSLQLQ
KERGSAHQRHYSGRSMEL
Reference
[1] Nomura N et al (1996) Genetic organization of a DNA-processing region required for mobilization of a non-self-transmissible plasmid, pEC3, isolated from Erwinia carotovora subsp. carotovora. Gene. 170(1):57-62. [PMID:8621089]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 238 MobB_pEC3 experimental BAA08131 161 aa MbeB-like, N-term conserved region; pfam04837
2 239 MobC_pEC3 experimental BAA08129 114 aa Bacterial mobilisation protein (MobC); pfam05713
 N/A
Information of plasmid
ID 166
Plasmid name pEC3
GenBank accession number D45188.1
Genome size 5.8 kb
Coordinate of oriT  [Strand] 797..804 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Erwinia carotovora IFO3380 [554]
Reference
 N/A